Summary of Study ST000671

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000474. The data can be accessed directly via it's Project DOI: 10.21228/M8SG7H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000671
Study TitleLCR/HCR rat mitochondrial study
Study TypeMS analysis
Study SummaryLCR and HCR rats (from Nathan Qi's 2010 study) were dissected at rest or following 10 minutes of exercise. Mitochondria were isolated from frozen gastrocnemius skeletal muscle. Samples consist of these isolated mitochondria suspended in 50 uL of mitochondrial isolation buffer (IBM containing sucrose, salts, etc; see Katie Overmyer's notebook E, p~23).
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups4
Total Subjects12
Study CommentsRecommend extracting entire sample suspension, NOT pelleting and removing supernatant. Quantities are likely to be small (~1-3mg tissue each?) Protein assays were performed to estimate quantities; see Katie's notebook or contact Charles for these protein concentration estimates, but also RECOMMEND SAVING RESIDUAL PELLET to allow further normalization.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SG7H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000474
Project DOI:doi: 10.21228/M8SG7H
Project Title:Lipidomics of LCR/HCR rat mitochondria
Project Type:MS analysis
Project Summary:Lipidomics of LCR/HCR rat mitochondria (2010 study, rest and 10min exercise)
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Burant Lab
Last Name:Burant
First Name:Chuck
Address:1500 E. Medical Center Drive Ann Arbor, MI 48109
Email:burantc@umich.edu
Phone:734 615-3481

Subject:

Subject ID:SU000694
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Line Rest/Exercise
SA038564S00021228HCR 10 minutes Exercise
SA038565S00021226HCR 10 minutes Exercise
SA038566S00021227HCR 10 minutes Exercise
SA038567S00021224HCR Rest
SA038568S00021223HCR Rest
SA038569S00021225HCR Rest
SA038570S00021232LCR 10 minutes Exercise
SA038571S00021234LCR 10 minutes Exercise
SA038572S00021233LCR 10 minutes Exercise
SA038573S00021229LCR Rest
SA038574S00021230LCR Rest
SA038575S00021231LCR Rest
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000688
Collection Summary:-
Sample Type:Mitochondria

Treatment:

Treatment ID:TR000708
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000701
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001028 AN001029
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000734
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000923
Analysis ID:AN001028
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000924
Analysis ID:AN001029
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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