Summary of Study ST001208

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000812. The data can be accessed directly via it's Project DOI: 10.21228/M8QM44 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001208
Study TitleCharacterization of feces in Atrial Fibrillation (AF) patients
Study SummaryAtrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid and irregular beating of the atria, is the most common arrhythmia with heavy global burdens. The present project aimed to characterized the feature of metabolites in feces of AF patients by using LC-MS.
Institute
Capital Medical University
Last NameZuo
First NameKun
Addressbeijing chaoyang hospital
Emailzuokun699@163.com
Phone010-85231937
Submit Date2018-12-27
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Kun Zuo Kun Zuo
https://dx.doi.org/10.21228/M8QM44
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000812
Project DOI:doi: 10.21228/M8QM44
Project Title:Characterization of feces in Atrial Fibrillation (AF) patients
Project Summary:Atrial Fibrillation (AF), an abnormal heart rhythm characterized by the rapid and irregular beating of the atria, is the most common arrhythmia with heavy global burdens. The present project aimed to characterized the feature of metabolites in feces of AF patients.
Institute:Capital Medical University
Last Name:Zuo
First Name:Kun
Address:beijing chaoyang hospital
Email:zuokun699@163.com
Phone:+861085231937

Subject:

Subject ID:SU001275
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Factors
SA084822AF37AF
SA084823AF38AF
SA084824AF40AF
SA084825AF36AF
SA084826AF39AF
SA084827AF35AF
SA084828AF32AF
SA084829AF33AF
SA084830AF34AF
SA084831AF41AF
SA084832AF42AF
SA084833AF48AF
SA084834AF49AF
SA084835AF50AF
SA084836AF47AF
SA084837AF46AF
SA084838AF43AF
SA084839AF44AF
SA084840AF45AF
SA084841AF31AF
SA084842AF30AF
SA084843AF15AF
SA084844AF16AF
SA084845AF17AF
SA084846AF18AF
SA084847AF14AF
SA084848AF13AF
SA084849AF10AF
SA084850AF11AF
SA084851AF12AF
SA084852AF19AF
SA084853AF20AF
SA084854AF26AF
SA084855AF27AF
SA084856AF28AF
SA084857AF29AF
SA084858AF25AF
SA084859AF24AF
SA084860AF21AF
SA084861AF22AF
SA084862AF23AF
SA084863AF9AF
Showing results 1 to 42 of 42

Collection:

Collection ID:CO001269
Collection Summary:Fresh stool samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C.
Sample Type:Feces

Treatment:

Treatment ID:TR001290
Treatment Summary:We enrolled participants consisting of patients with AF.

Sample Preparation:

Sampleprep ID:SP001283
Sampleprep Summary:20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.

Combined analysis:

Analysis ID AN002010 AN002011
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Hypersil Gold C18 Hypersil Gold C18
MS Type ESI ESI
MS instrument type Ion trap Ion trap
MS instrument name Thermo Deca LCQ Thermo Deca LCQ
Ion Mode POSITIVE NEGATIVE
Units pg pg

Chromatography:

Chromatography ID:CH001454
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Hypersil Gold C18
Chromatography Type:Reversed phase

MS:

MS ID:MS001863
Analysis ID:AN002010
Instrument Name:Thermo Deca LCQ
Instrument Type:Ion trap
MS Type:ESI
MS Comments:Compound Discoverer 2.0 software (Thermo)
Ion Mode:POSITIVE
  
MS ID:MS001864
Analysis ID:AN002011
Instrument Name:Thermo Deca LCQ
Instrument Type:Ion trap
MS Type:ESI
MS Comments:Compound Discoverer 2.0 software (Thermo)
Ion Mode:NEGATIVE
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