Summary of Study ST001620
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001620 |
| Study Title | Dietary composition analysis of chow diet and purified diet using untargeted metabonomics |
| Study Summary | Dietary patterns and psychosocial factors, ubiquitous part of modern lifestyle, critically shape the gut microbiota and human health. However, it remains obscure how dietary and psychosocial inputs coordinately modulate the gut microbiota and host impact. Here, we show that dietary raffinose metabolism to fructose couples stress-induced gut microbial remodeling to intestinal stem cells (ISC) renewal and epithelial homeostasis. Chow diet (CD) and purified diet (PD) confer distinct vulnerability to gut epithelial injury, microbial alternation and ISC dysfunction in chronically restrained mice. CD preferably enriches Lactobacillus reuteri, and its colonization is sufficient to rescue stress-triggered epithelial injury. |
| Institute | China Pharmaceutical University |
| Department | School of Medicine |
| Laboratory | metabonomics |
| Last Name | Yuanlong |
| First Name | Hou |
| Address | Nanjing, Jiangsu |
| jian2103@163.com | |
| Phone | 86-18851105337 |
| Submit Date | 2020-12-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-12-16 |
| Release Version | 1 |
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Project:
| Project ID: | PR001041 |
| Project DOI: | doi: 10.21228/M84M6M |
| Project Title: | Dietary composition analysis studies |
| Project Type: | Untargeted metabolomics analysis with two diets |
| Project Summary: | Dietary patterns and psychosocial factors, ubiquitous part of modern lifestyle, critically shape the gut microbiota and human health. However, it remains obscure how dietary and psychosocial inputs coordinately modulate the gut microbiota and host impact. Here, we show that dietary raffinose metabolism to fructose couples stress-induced gut microbial remodeling to intestinal stem cells (ISC) renewal and epithelial homeostasis. Chow diet (CD) and purified diet (PD) confer distinct vulnerability to gut epithelial injury, microbial alternation and ISC dysfunction in chronically restrained mice. CD preferably enriches Lactobacillus reuteri, and its colonization is sufficient to rescue stress-triggered epithelial injury. |
| Institute: | China Pharmaceutical University |
| Department: | School of Medicine |
| Laboratory: | metabonomics |
| Last Name: | Yunalong |
| First Name: | Hou |
| Address: | Nanjing, Jiangsu |
| Email: | jian2103@163.com |
| Phone: | 18851105337 |
Subject:
| Subject ID: | SU001697 |
| Subject Type: | Food item |
| Subject Species: | - |
Factors:
Subject type: Food item; Subject species: - (Factor headings shown in green)
| mb_sample_id | local_sample_id | Diet |
|---|---|---|
| SA137908 | CD2 | Chow |
| SA137909 | CD3 | Chow |
| SA137910 | CD1 | Chow |
| SA137911 | PD4 | Purified |
| SA137912 | PD2 | Purified |
| SA137913 | PD3 | Purified |
| SA137914 | PD1 | Purified |
| Showing results 1 to 7 of 7 |
Collection:
| Collection ID: | CO001690 |
| Collection Summary: | Purified diet and chow diet |
| Sample Type: | Diet |
| Storage Conditions: | 4℃ |
Treatment:
| Treatment ID: | TR001710 |
| Treatment Summary: | Diet pellets (30 mg) were weighted by adding 400 µL of methanol (pre-cooled at -20℃), vortexed for 60 s, and then centrifuged for 10 min at 13,000 g, 4℃. The supernatant was evaporated to dryness under vacuum, reconstituted with 150 μL of 2-chlorophenyalanine (4ppm) methanol aqueous solution. |
Sample Preparation:
| Sampleprep ID: | SP001703 |
| Sampleprep Summary: | Diet pellets (30 mg) come from lab |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | 4℃ |
Combined analysis:
| Analysis ID | AN002655 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UltiMate 3000 |
| Column | Waters Acquity UPLC HSS T3 (150x2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus |
| Ion Mode | UNSPECIFIED |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001958 |
| Instrument Name: | UltiMate 3000 |
| Column Name: | Waters Acquity UPLC HSS T3 (150x2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002462 |
| Analysis ID: | AN002655 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data dependent acquisition The Progenesis QI MetaboAnalyst 3.0 |
| Ion Mode: | UNSPECIFIED |
