Summary of Study ST001774
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001128. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ4Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001774 |
Study Title | Timecourse on primary human highly differentiated airway epithelial cells infected with HRV-C15 |
Study Summary | At each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
Institute | McGill University |
Last Name | Lopes |
First Name | Fernando |
Address | 21111 Lakeshore Rd |
fernando.lopes@mcgill.ca | |
Phone | 5143987607 |
Submit Date | 2021-05-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-12 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001128 |
Project DOI: | doi: 10.21228/M8WQ4Q |
Project Title: | PGC-1 alpha mediates a metabolic host defense response in human airway epithelium during rhinovirus infections |
Project Summary: | Quantitative Metabolomics studies on samples from human airway epithelial cell cultures |
Institute: | McGill University |
Last Name: | Fernando Lopes |
First Name: | Fernando |
Address: | 21111 Lakeshore Rd, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada |
Email: | fernando.lopes@mcgill.ca |
Phone: | 5143987607 |
Subject:
Subject ID: | SU001851 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Experimental variable |
---|---|---|
SA164523 | 2020_04_15_AM_HLICNeg15_HBE84_12_C | Control 12h |
SA164524 | 2020_04_15_AM_HLICNeg15_HBE85_12_C | Control 12h |
SA164525 | 2020_04_15_AM_HLICNeg15_HBE91_4_C | Control 12h |
SA164526 | 2020_04_15_AM_HLICNeg15_HBE82_12_C | Control 12h |
SA164527 | 2020_04_15_AM_HLICNeg15_HBE90_12_C | Control 12h |
SA164528 | 2020_04_15_AM_HLICNeg15_HBE58_12_C | Control 12h |
SA164529 | 2020_04_15_AM_HLICNeg15_HBE85_24_C | Control 24h |
SA164530 | 2020_04_15_AM_HLICNeg15_HBE91_12_C | Control 24h |
SA164531 | 2020_04_15_AM_HLICNeg15_HBE58_24_C | Control 24h |
SA164532 | 2020_04_15_AM_HLICNeg15_HBE90_24_C | Control 24h |
SA164533 | 2020_04_15_AM_HLICNeg15_HBE84_24_C | Control 24h |
SA164534 | 2020_04_15_AM_HLICNeg15_HBE82_24_C | Control 24h |
SA164535 | 2020_04_15_AM_HLICNeg15_HBE82_4_C | Control 4h |
SA164536 | 2020_04_15_AM_HLICNeg15_HBE91_24_C | Control 4h |
SA164537 | 2020_04_15_AM_HLICNeg15_HBE90_4_C | Control 4h |
SA164538 | 2020_04_15_AM_HLICNeg15_HBE84_4_C | Control 4h |
SA164539 | 2020_04_15_AM_HLICNeg15_HBE58_4_C | Control 4h |
SA164540 | 2020_04_15_AM_HLICNeg15_HBE85_4_C | Control 4h |
SA164541 | 2020_04_15_AM_HLICNeg15_HBE84_12_V | HRV-C15 infection 12h |
SA164542 | 2020_04_15_AM_HLICNeg15_HBE90_12_V | HRV-C15 infection 12h |
SA164543 | 2020_04_15_AM_HLICNeg15_Media_12_V | HRV-C15 infection 12h |
SA164544 | 2020_04_15_AM_HLICNeg15_HBE58_12_V | HRV-C15 infection 12h |
SA164545 | 2020_04_15_AM_HLICNeg15_HBE85_12_V | HRV-C15 infection 12h |
SA164546 | 2020_04_15_AM_HLICNeg15_HBE91_12_V | HRV-C15 infection 12h |
SA164547 | 2020_04_15_AM_HLICNeg15_HBE82_12_V | HRV-C15 infection 12h |
SA164548 | 2020_04_15_AM_HLICNeg15_HBE85_24_V | HRV-C15 infection 24h |
SA164549 | 2020_04_15_AM_HLICNeg15_HBE82_24_V | HRV-C15 infection 24h |
SA164550 | 2020_04_15_AM_HLICNeg15_HBE84_24_V | HRV-C15 infection 24h |
SA164551 | 2020_04_15_AM_HLICNeg15_HBE91_24_V | HRV-C15 infection 24h |
SA164552 | 2020_04_15_AM_HLICNeg15_Media_24_V | HRV-C15 infection 24h |
SA164553 | 2020_04_15_AM_HLICNeg15_HBE58_24_V | HRV-C15 infection 24h |
SA164554 | 2020_04_15_AM_HLICNeg15_HBE90_24_V | HRV-C15 infection 24h |
SA164555 | 2020_04_15_AM_HLICNeg15_HBE91_4_V | HRV-C15 infection 4h |
SA164556 | 2020_04_15_AM_HLICNeg15_HBE90_4_V | HRV-C15 infection 4h |
SA164557 | 2020_04_15_AM_HLICNeg15_HBE58_4_V | HRV-C15 infection 4h |
SA164558 | 2020_04_15_AM_HLICNeg15_HBE85_4_V | HRV-C15 infection 4h |
SA164559 | 2020_04_15_AM_HLICNeg15_Media_4_V | HRV-C15 infection 4h |
SA164560 | 2020_04_15_AM_HLICNeg15_HBE82_4_V | HRV-C15 infection 4h |
SA164561 | 2020_04_15_AM_HLICNeg15_HBE84_4_V | HRV-C15 infection 4h |
SA164562 | 2020_04_15_AM_HLICNeg15_Media_F | Media |
SA164563 | 2020_04_15_AM_HLICNeg15_Media_12 | Media 12h in the incubator |
SA164564 | 2020_04_15_AM_HLICNeg15_Media_24 | Media 24h in the incubator |
SA164565 | 2020_04_15_AM_HLICNeg15_Media_4 | Media 4h in the incubator |
SA164566 | 2020_04_15_AM_HLICNeg15_Midblank1 | Midblank |
SA164567 | 2020_04_15_AM_HLICNeg15_Postplank1 | Postplan |
SA164568 | 2020_04_15_AM_HLICNeg15_Postplank3 | Postplan |
SA164569 | 2020_04_15_AM_HLICNeg15_Postplank2 | Postplan |
SA164570 | 2020_04_15_AM_HLICNeg15_Preblank1 | Preblank |
SA164571 | 2020_04_15_AM_HLICNeg15_Preblank2 | Preblank |
SA164572 | 2020_04_15_AM_HLICNeg15_Preblank3 | Preblank |
Showing results 1 to 50 of 50 |
Collection:
Collection ID: | CO001844 |
Collection Summary: | At each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001864 |
Treatment Summary: | Highly differentiated primary human airway epithelial were infected or not with HRV-C15 for 4, 12 or 24 h. |
Treatment Compound: | Human rhinovirus C15 |
Sample Preparation:
Sampleprep ID: | SP001857 |
Sampleprep Summary: | media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
Combined analysis:
Analysis ID | AN002881 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | pmoles/l |
Chromatography:
Chromatography ID: | CH002136 |
Chromatography Summary: | General metabolomic run were performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (ThermoFisher) coupled to a Vanquish™ UHPLC System (ThermoFisher). Chromatographic separation was achieved on a Syncronis HILIC UHPLC column (2.1 mm x 100 mm x 1.7 mm, ThermoFisher) using a binary solvent system at a flow rate of 600 mL/min. Solvent A, 20 mM ammonium formate pH 3.0 in mass spectrometry grade H2O; Solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (% v/v). A sample injection volume of 2 mL was used. |
Instrument Name: | Thermo Vanquish |
Column Name: | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
Solvent A: | 100% water; 20 mM ammonium formate, pH 3 |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002674 |
Analysis ID: | AN002881 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | The mass spectrometer was run in negative full scan mode at a resolution of 240,000 scanning from 50-750 m/z. |
Ion Mode: | NEGATIVE |