Summary of Study ST001885

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001188. The data can be accessed directly via it's Project DOI: 10.21228/M84X4Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001885
Study TitleMS Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome
Study SummaryRecognition and rejection of chemically defended prey is critical to maximizing fitness for predators. Paralytic shellfish toxins (PSTs) which strongly inhibit voltage-gated sodium channels in diverse animal taxa are produced by several species of the bloom-forming algal genus Alexandrium where they appear to function as chemical defenses against grazing copepods. Despite PSTs being produced and localized within phytoplankton cells, some copepods distinguish toxic from non-toxic prey, selectively ingesting less toxic cells, in ways that suggest cell surface recognition perhaps associated with non-polar metabolites. In this study LC/MS and NMR-based metabolomics revealed that the non-polar metabolomes of two toxic species (Alexandrium catenella and Alexandrium pacificum) vary considerably from their non-toxic congener Alexandrium tamarense despite all three being very closely related. Toxic and non-toxic Alexandrium spp. were distinguished from each other by metabolites belonging to seven lipid classes. Of these, 17 specific metabolites were significantly more abundant in both toxic A. catenella and A. pacificum compared to non-toxic A. tamarense suggesting that just a small portion of the observed metabolic variability is associated with toxicity. Future experiments aimed at deciphering chemoreception mechanisms of copepod perception of Alexandrium toxicity should consider these metabolites, and the broader lipid classes phosphatidylcholines and sterols, as potential candidate cues.
Institute
Georgia Institute of Technology
Last NameBrown
First NameEmily
Address950 Atlantic Dr Atlanta GA 30332, USA
Emailjulia.kubanek@biosci.gatech.edu
Phone404-894-8424
Submit Date2021-07-22
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-08-09
Release Version1
Emily Brown Emily Brown
https://dx.doi.org/10.21228/M84X4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001188
Project DOI:doi: 10.21228/M84X4Z
Project Title:Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome
Project Summary:Recognition and rejection of chemically defended prey is critical to maximizing fitness for predators. Paralytic shellfish toxins (PSTs) which strongly inhibit voltage-gated sodium channels in diverse animal taxa are produced by several species of the bloom-forming algal genus Alexandrium where they appear to function as chemical defenses against grazing copepods. Despite PSTs being produced and localized within phytoplankton cells, some copepods distinguish toxic from non-toxic prey, selectively ingesting less toxic cells, in ways that suggest cell surface recognition perhaps associated with non-polar metabolites. In this study LC/MS and NMR-based metabolomics revealed that the non-polar metabolomes of two toxic species (Alexandrium catenella and Alexandrium pacificum) vary considerably from their non-toxic congener Alexandrium tamarense despite all three being very closely related. Toxic and non-toxic Alexandrium spp. were distinguished from each other by metabolites belonging to seven lipid classes. Of these, 17 specific metabolites were significantly more abundant in both toxic A. catenella and A. pacificum compared to non-toxic A. tamarense suggesting that just a small portion of the observed metabolic variability is associated with toxicity. Future experiments aimed at deciphering chemoreception mechanisms of copepod perception of Alexandrium toxicity should consider these metabolites, and the broader lipid classes phosphatidylcholines and sterols, as potential candidate cues.
Institute:Georgia Institute of Technology
Last Name:Brown
First Name:Emily
Address:950 Atlantic Dr Atlanta GA 30332, USA
Email:julia.kubanek@biosci.gatech.edu
Phone:404-894-8424

Subject:

Subject ID:SU001963
Subject Type:Other organism
Subject Species:Alexandrium catenella;Alexandrium tamarense;Alexandrium pacificum
Taxonomy ID:2925;2926;1565494
Species Group:Alexandrium catenella; Alexandrium tamarense; Alexandrium pacificum

Factors:

Subject type: Other organism; Subject species: Alexandrium catenella;Alexandrium tamarense;Alexandrium pacificum (Factor headings shown in green)

mb_sample_id local_sample_id factor
SA175199QC06QC
SA175200QC07QC
SA175201QC08QC
SA175202QC10QC
SA175203QC05QC
SA175204QC03QC
SA175205Blank01QC
SA175206Blank02QC
SA175207QC01QC
SA175208QC02QC
SA175209QC04QC
SA175210QC09QC
SA17521174_3ERB74_AC_14Treatment;Alexandrium_catenella
SA17521274_3ERB74_AC_7Treatment;Alexandrium_catenella
SA17521374_3ERB74_AC_5Treatment;Alexandrium_catenella
SA17521474_3ERB74_AC_4Treatment;Alexandrium_catenella
SA17521574_3ERB74_AC_2Treatment;Alexandrium_catenella
SA17521674_3ERB74_AC_3Treatment;Alexandrium_catenella
SA17521774_3ERB74_AC_8Treatment;Alexandrium_catenella
SA17521874_3ERB74_AC_6Treatment;Alexandrium_catenella
SA17521974_3ERB74_AC_13Treatment;Alexandrium_catenella
SA17522074_3ERB74_AC_9Treatment;Alexandrium_catenella
SA17522174_3ERB74_AC_12Treatment;Alexandrium_catenella
SA17522274_3ERB74_AC_15Treatment;Alexandrium_catenella
SA17522374_3ERB74_AC_1Treatment;Alexandrium_catenella
SA17522474_3ERB74_AC_10Treatment;Alexandrium_catenella
SA17522574_3ERB74_AC_11Treatment;Alexandrium_catenella
SA17522675_3ERB75_AP_9Treatment;Alexandrium_pacificum
SA17522775_3ERB75_AP_7Treatment;Alexandrium_pacificum
SA17522875_3ERB75_AP_10Treatment;Alexandrium_pacificum
SA17522975_3ERB75_AP_14Treatment;Alexandrium_pacificum
SA17523075_3ERB75_AP_15Treatment;Alexandrium_pacificum
SA17523175_3ERB75_AP_13Treatment;Alexandrium_pacificum
SA17523275_3ERB75_AP_11Treatment;Alexandrium_pacificum
SA17523375_3ERB75_AP_6Treatment;Alexandrium_pacificum
SA17523475_3ERB75_AP_1Treatment;Alexandrium_pacificum
SA17523575_3ERB75_AP_5Treatment;Alexandrium_pacificum
SA17523675_3ERB75_AP_2Treatment;Alexandrium_pacificum
SA17523775_3ERB75_AP_3Treatment;Alexandrium_pacificum
SA17523875_3ERB75_AP_4Treatment;Alexandrium_pacificum
SA17523974_3ERB74_AT_10Treatment;Alexandrium_tamarense
SA17524074_3ERB74_AT_8Treatment;Alexandrium_tamarense
SA17524174_3ERB74_AT_9Treatment;Alexandrium_tamarense
SA17524274_3ERB74_AT_13Treatment;Alexandrium_tamarense
SA17524374_3ERB74_AT_7Treatment;Alexandrium_tamarense
SA17524474_3ERB74_AT_12Treatment;Alexandrium_tamarense
SA17524574_3ERB74_AT_11Treatment;Alexandrium_tamarense
SA17524674_3ERB74_AT_4Treatment;Alexandrium_tamarense
SA17524774_3ERB74_AT_1Treatment;Alexandrium_tamarense
SA17524874_3ERB74_AT_14Treatment;Alexandrium_tamarense
SA17524974_3ERB74_AT_15Treatment;Alexandrium_tamarense
SA17525074_3ERB74_AT_2Treatment;Alexandrium_tamarense
SA17525174_3ERB74_AT_3Treatment;Alexandrium_tamarense
SA17525274_3ERB74_AT_6Treatment;Alexandrium_tamarense
SA17525374_3ERB74_AT_5Treatment;Alexandrium_tamarense
SA17525475_3ERB75_AT_15Treatment;Alexandrium_tamarense2
SA17525575_3ERB75_AT_11Treatment;Alexandrium_tamarense2
SA17525675_3ERB75_AT_4Treatment;Alexandrium_tamarense2
SA17525775_3ERB75_AT_5Treatment;Alexandrium_tamarense2
SA17525875_3ERB75_AT_3Treatment;Alexandrium_tamarense2
SA17525975_3ERB75_AT_2Treatment;Alexandrium_tamarense2
SA17526075_3ERB75_AT_1Treatment;Alexandrium_tamarense2
SA17526175_3ERB75_AT_6Treatment;Alexandrium_tamarense2
SA17526275_3ERB75_AT_7Treatment;Alexandrium_tamarense2
SA17526375_3ERB75_AT_12Treatment;Alexandrium_tamarense2
SA17526475_3ERB75_AT_13Treatment;Alexandrium_tamarense2
SA17526575_3ERB75_AT_10Treatment;Alexandrium_tamarense2
SA17526675_3ERB75_AT_9Treatment;Alexandrium_tamarense2
SA17526775_3ERB75_AT_8Treatment;Alexandrium_tamarense2
SA17526875_3ERB75_AT_14Treatment;Alexandrium_tamarense2
Showing results 1 to 70 of 70

Collection:

Collection ID:CO001956
Collection Summary:Alexandrium cells were collected by vacuum filtration onto GF/F filters and quenched with liquid nitrogen. Frozen cells with filters were stored in foil(previously muffled for 3 h at 450 °C) at -80 °C until extraction.
Sample Type:Algae

Treatment:

Treatment ID:TR001975
Treatment Summary:Metabolomes of toxic versus non-toxic species were compared using the following experimental pairings: A. tamarense (n=15) with A. catenella (n=15) (Experiment 1) and A. tamarense (n=15) with A. pacificum (n=15) (Experiment 2). The same non-toxic strain of A. tamarense was used in both experiments but the two experiments were conducted separately, in different months, to make the experiment manageable based on availability of batches grown from stock cultures. For both experiments, Alexandrium spp. cultures at a cell density of 12,000 to 13,000 cells mL-1 were split into fifteen 300 mL subcultures of each species which grew for two days. At the end of each experiment, during harvesting, a 1.0 mL aliquot from each culture was preserved with Lugol’s solution to measure cell concentrations

Sample Preparation:

Sampleprep ID:SP001969
Sampleprep Summary:IPA: ACN (2:1) extract 4.54e6 cells per 1mL

Combined analysis:

Analysis ID AN003049 AN003050
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um)
MS Type ESI ESI
MS instrument type orbitrap and ion trap orbitrap and ion trap
MS instrument name Thermo Orbitrap ID-X tribrid Thermo Orbitrap ID-X tribrid
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH002259
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002836
Analysis ID:AN003049
Instrument Name:Thermo Orbitrap ID-X tribrid
Instrument Type:orbitrap and ion trap
MS Type:ESI
MS Comments:compound discoverer
Ion Mode:POSITIVE
  
MS ID:MS002837
Analysis ID:AN003050
Instrument Name:Thermo Orbitrap ID-X tribrid
Instrument Type:orbitrap and ion trap
MS Type:ESI
MS Comments:compound discoverer
Ion Mode:NEGATIVE
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