Summary of Study ST001941

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001229. The data can be accessed directly via it's Project DOI: 10.21228/M8V98S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001941
Study TitleUntargeted metabolomics of breast cell lines in the presence or the absence of CtBP inhibitors
Study SummaryExperiments to test the effect of CtBP2 inhibition on metabolism of breast cell lines. In particular, experiment 1 involves comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231). Experiment 2 is a study between MDA-MB231 silenced for CtBP2 by stable RNA interference (shCtBP2 cells) compared to scramble (shCTRL cells). Experiment 3 is a comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231)in the presence of the absence of small-molecule CtBP inhibitors: HIPP (400 μM) or P4 (300 μM)for 48 hours.
Institute
University of Milano-Bicocca
Last NameBonanomi
First NameMarcella
AddressPiazza della Scienza 4
Emailmarcella.bonanomi@unimib.it
Phone+390264483343
Submit Date2021-08-31
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2021-11-04
Release Version1
Marcella Bonanomi Marcella Bonanomi
https://dx.doi.org/10.21228/M8V98S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001229
Project DOI:doi: 10.21228/M8V98S
Project Title:Untargeted metabolomics of breast cell lines in the presence or the absence of CtBP inhibitors
Project Summary:Experiments to test the effect of CtBP2 inhibition on metabolism of breast cell lines
Institute:University of Milano-Bicocca
Last Name:Bonanomi
First Name:Marcella
Address:Piazza della Scienza 4, Milan, MI, 20126, Italy
Email:marcella.bonanomi@unimib.it
Phone:+390264483343

Subject:

Subject ID:SU002019
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id CELL LINE TREATMENT
SA18325510A CTRL 1_001MCF102A CONTROL
SA18325610A CTRL 1_1MCF102A CONTROL
SA18325710A CTRL 1_3MCF102A CONTROL
SA18325810A CTRL 2_1MCF102A CONTROL
SA18325910A CTRL 2_2MCF102A CONTROL
SA18326010A CTRL 1_2MCF102A CONTROL
SA18326110A CTRL 2_3MCF102A CONTROL
SA18326210A CTRL 1_003MCF102A CONTROL
SA18326310A CTRL 2_001MCF102A CONTROL
SA18326410A CTRL 2_002MCF102A CONTROL
SA18326510A CTRL 2_003MCF102A CONTROL
SA18326610A CTRL 1_002MCF102A CONTROL
SA18326710A HIPP 2_2MCF102A HIPP
SA18326810A HIPP 2_3MCF102A HIPP
SA18326910A HIPP 1_3MCF102A HIPP
SA18327010A HIPP 1_2MCF102A HIPP
SA18327110A HIPP 1_1MCF102A HIPP
SA18327210A HIPP 2_1MCF102A HIPP
SA18327310A P4 2_2MCF102A P4
SA18327410A P4 1_2MCF102A P4
SA18327510A P4 2_1MCF102A P4
SA18327610A P4 1_3MCF102A P4
SA18327710A P4 1_1MCF102A P4
SA18327810A P4 2_3MCF102A P4
SA183279231 CTRL 1_3MDA-MB231 CONTROL
SA183280231 CTRL 1_001MDA-MB231 CONTROL
SA183281231 CTRL 2_3MDA-MB231 CONTROL
SA183282231 CTRL 2_2MDA-MB231 CONTROL
SA183283231 CTRL 2_1MDA-MB231 CONTROL
SA183284231 CTRL 1_2MDA-MB231 CONTROL
SA183285231 CTRL 1_1MDA-MB231 CONTROL
SA183286231 CTRL 2_003MDA-MB231 CONTROL
SA183287231 CTRL 1_002MDA-MB231 CONTROL
SA183288231 CTRL 2_002MDA-MB231 CONTROL
SA183289231 CTRL 1_003MDA-MB231 CONTROL
SA183290231 CTRL 2_001MDA-MB231 CONTROL
SA183291231 HIPP 1_3MDA-MB231 HIPP
SA183292231 HIPP 2_1MDA-MB231 HIPP
SA183293231 HIPP 2_3MDA-MB231 HIPP
SA183294231 HIPP 1_1MDA-MB231 HIPP
SA183295231 HIPP 2_2MDA-MB231 HIPP
SA183296231 HIPP 1_2MDA-MB231 HIPP
SA183297231 P4 1_3MDA-MB231 P4
SA183298231 P4 2_1MDA-MB231 P4
SA183299231 P4 2_2MDA-MB231 P4
SA183300231 P4 2_3MDA-MB231 P4
SA183301231 P4 1_2MDA-MB231 P4
SA183302231 P4 1_1MDA-MB231 P4
SA183303sh CTRL_003MDA-MB231 SCRAMBLE
SA183304sh CTRL_002MDA-MB231 SCRAMBLE
SA183305sh CTRL_001MDA-MB231 SCRAMBLE
SA183306shCtBP2_002MDA-MB231 shCtBP2
SA183307shCtBP2_003MDA-MB231 shCtBP2
SA183308shCtBP2_001MDA-MB231 shCtBP2
Showing results 1 to 54 of 54

Collection:

Collection ID:CO002012
Collection Summary:MDA-MB231 and MCF102A cell lines were obtained from the American Type Culture Collection (ATCC) (LGC Standard, UK). MDA-MB231 cell line was grown in Dulbecco's modified Eagle's medium (DMEM) containing 4mM L-glutamine, supplemented with 10% fetal bovine serum. MCF102A cell line was maintained in DMEM/F-12 containing 5% horse serum, 2.5mM L-glutamine, 20ng/ml EGF, 100 ng/ml cholera toxin, 0.01 mg/ml insulin, and 500 ng/ml hydrocortisone. All media were supplemented with 100 U/ml penicillin and 100 µg/ml streptomycin, and cells were incubated at 37°C in a 5% CO2 incubator.
Sample Type:Breast cancer cells

Treatment:

Treatment ID:TR002031
Treatment Summary:cells were plated in 6-well plates with normal growth medium and then incubated for 48h in a complete fresh medium in the presence or absence of treatments (HIPP 400 μM or P4 300 μM)

Sample Preparation:

Sampleprep ID:SP002025
Sampleprep Summary:Cells were quickly rinsed with NaCl 0.9% and quenched with 500μl ice-cold 70:30 acetonitrile: water. Plates were placed at -80°C for 10 minutes, collected by scraping, sonicated 5 sec for five pulses at 70% power twice, and then centrifuged at 12000g for 10 min at 4°C. The supernatant was collected in a glass insert and evaporated under airflow at 37°C. Samples were then resuspended with 150 μl of H2O before analyses.

Combined analysis:

Analysis ID AN003159
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm,2.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6550 QTOF
Ion Mode NEGATIVE
Units Area

Chromatography:

Chromatography ID:CH002337
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm,2.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002939
Analysis ID:AN003159
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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