Summary of Study ST002050

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001296. The data can be accessed directly via it's Project DOI: 10.21228/M86697 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002050
Study TitlePerfluoroalkyl Compounds and Child Metabolic Health (Project Viva)
Study SummaryProject Viva: Pregnant women were enrolled in Project Viva between 1999 and 2002 at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard Vanguard Medical Associates, a multispecialty group practice in eastern Massachusetts. Eligible mothers were fluent in English, had singleton gestations, were <22 weeks gestation, and had no plans to move away from the study area. Research staff performed in-person study visits with participating mothers in the first (median gestational age 9.9 weeks) and second (median gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and children during the first few days after delivery, during infancy (median age 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median age 7.7 years), and adolescence (median age13 years). In this analysis, we will use data from 188 mother-child pairs in Project Viva with available information on prenatal PFAS concentrations, available umbilical cord serum samples at delivery, and outcomes of interest. Please contact Nicole Bornkamp at Nicole_Bornkamp@harvardpilgrim.org for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Project Viva is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Parker CB; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Harvard Pilgrim Health Care Institute
DepartmentPopulation Medicine
LaboratoryChanning
Last NameOken
First NameEmily
Address401 Park Drive Ste 401, Boston, MA 02215
Emailemily_oken@harvardpilgrim.org
Phone617-867-4835
Submit Date2021-12-23
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-12-23
Release Version1
Emily Oken Emily Oken
https://dx.doi.org/10.21228/M86697
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001296
Project DOI:doi: 10.21228/M86697
Project Title:Perfluoroalkyl Compounds and Child Metabolic Health (Healthy Start Cohort)
Project Type:Metabolomics
Project Summary:The project Perfluoroalkyl Compounds and Child Metabolic Health is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from the Healthy Start and Project Viva ECHO cohorts. This project will examine associations of untargeted metabolomics data acquired from cord blood with prenatal PFAS exposure and the effect on infant/child metabolic health.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner/Li Lab
Last Name:Sumner, Li
First Name:Susan, Yuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:susan_sumner@unc.edu, yuanyli4@unc.edu
Phone:9196224456
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Healthy Start is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Parker CB; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002132
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Study type
SA193276HHEAR_10_OkenHHEAR reference material
SA193277HHEAR_9_OkenHHEAR reference material
SA193278HHEAR_11_OkenHHEAR reference material
SA193279HHEAR_12_OkenHHEAR reference material
SA193280HHEAR_13_OkenHHEAR reference material
SA193281HHEAR_8_OkenHHEAR reference material
SA193282HHEAR_6_OkenHHEAR reference material
SA193283HHEAR_2_OkenHHEAR reference material
SA193284HHEAR_1_OkenHHEAR reference material
SA193285HHEAR_3_OkenHHEAR reference material
SA193286HHEAR_4_OkenHHEAR reference material
SA193287HHEAR_14_OkenHHEAR reference material
SA193288HHEAR_7_OkenHHEAR reference material
SA193289HHEAR_5_OkenHHEAR reference material
SA193290HHEAR_20_OkenHHEAR reference material
SA193291HHEAR_15_OkenHHEAR reference material
SA193292HHEAR_18_OkenHHEAR reference material
SA193293HHEAR_19_OkenHHEAR reference material
SA193294HHEAR_17_OkenHHEAR reference material
SA193295HHEAR_16_OkenHHEAR reference material
SA193296NIST_5_OkenNIST reference material
SA193297NIST_6_OkenNIST reference material
SA193298NIST_3_OkenNIST reference material
SA193299NIST_4_OkenNIST reference material
SA193300NIST_2_OkenNIST reference material
SA193301NIST_1_OkenNIST reference material
SA193302S_135_OkenStudy sample
SA193303S_136_OkenStudy sample
SA193304S_137_OkenStudy sample
SA193305S_138_OkenStudy sample
SA193306S_134_OkenStudy sample
SA193307S_133_OkenStudy sample
SA193308S_131_OkenStudy sample
SA193309S_132_OkenStudy sample
SA193310S_139_OkenStudy sample
SA193311S_130_OkenStudy sample
SA193312S_146_OkenStudy sample
SA193313S_147_OkenStudy sample
SA193314S_148_OkenStudy sample
SA193315S_129_OkenStudy sample
SA193316S_145_OkenStudy sample
SA193317S_144_OkenStudy sample
SA193318S_141_OkenStudy sample
SA193319S_142_OkenStudy sample
SA193320S_143_OkenStudy sample
SA193321S_140_OkenStudy sample
SA193322S_115_OkenStudy sample
SA193323S_149_OkenStudy sample
SA193324S_116_OkenStudy sample
SA193325S_117_OkenStudy sample
SA193326S_114_OkenStudy sample
SA193327S_113_OkenStudy sample
SA193328S_110_OkenStudy sample
SA193329S_111_OkenStudy sample
SA193330S_112_OkenStudy sample
SA193331S_1_OkenStudy sample
SA193332S_119_OkenStudy sample
SA193333S_125_OkenStudy sample
SA193334S_126_OkenStudy sample
SA193335S_127_OkenStudy sample
SA193336S_124_OkenStudy sample
SA193337S_123_OkenStudy sample
SA193338S_120_OkenStudy sample
SA193339S_121_OkenStudy sample
SA193340S_122_OkenStudy sample
SA193341S_128_OkenStudy sample
SA193342S_163_OkenStudy sample
SA193343S_176_OkenStudy sample
SA193344S_177_OkenStudy sample
SA193345S_178_OkenStudy sample
SA193346S_175_OkenStudy sample
SA193347S_174_OkenStudy sample
SA193348S_171_OkenStudy sample
SA193349S_172_OkenStudy sample
SA193350S_173_OkenStudy sample
SA193351S_179_OkenStudy sample
SA193352S_180_OkenStudy sample
SA193353S_186_OkenStudy sample
SA193354S_187_OkenStudy sample
SA193355S_188_OkenStudy sample
SA193356S_185_OkenStudy sample
SA193357S_184_OkenStudy sample
SA193358S_181_OkenStudy sample
SA193359S_182_OkenStudy sample
SA193360S_183_OkenStudy sample
SA193361S_170_OkenStudy sample
SA193362S_169_OkenStudy sample
SA193363S_156_OkenStudy sample
SA193364S_157_OkenStudy sample
SA193365S_158_OkenStudy sample
SA193366S_155_OkenStudy sample
SA193367S_154_OkenStudy sample
SA193368S_151_OkenStudy sample
SA193369S_152_OkenStudy sample
SA193370S_153_OkenStudy sample
SA193371S_159_OkenStudy sample
SA193372S_160_OkenStudy sample
SA193373S_166_OkenStudy sample
SA193374S_167_OkenStudy sample
SA193375S_168_OkenStudy sample
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Collection:

Collection ID:CO002125
Collection Summary:Pregnant women were enrolled in Project Viva between 1999 and 2002 at their first prenatal visit at one of 8 obstetric clinics of Atrius Harvard Vanguard Medical Associates, a multispecialty group practice in eastern Massachusetts. Eligible mothers were fluent in English, had singleton gestations, were <22 weeks gestation, and had no plans to move away from the study area. Research staff performed in-person study visits with participating mothers in the first (median gestational age 9.9 weeks) and second (median gestational age 28.1 weeks) trimesters of pregnancy, and with mothers and children during the first few days after delivery, during infancy (median age 6.3 months), in early childhood (median age 3.3 years), mid-childhood (median age 7.7 years), and adolescence (median age13 years).
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002144
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002138
Sampleprep Summary:Study plasma samples (50 µL) and total study pool samples (50 µL) were prepared and shipped from the Channing Laboratory Division of Network Medicine at Brigham and Women’s Hospital, Boston, MA, to the NC HHEAR Hub on dry ice. Aliquots of HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. All samples were stored under -80° C until lab analysis. All samples were thawed at 4 °C overnight a day before sample preparation. The same volume (50 µL) of LC-MS grade water was used as blank. Samples, including study samples, total study pool samples, HHEAR reference material, NIST reference material, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by the multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts, and the samples were thoroughly mixed on the multiple tube vortex mixer for 10 min at 5000 rpm in room temperature. All samples were centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003337
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Normalized intensity

Chromatography:

Chromatography ID:CH002471
Chromatography Summary:Reverse phase
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003106
Analysis ID:AN003337
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.1 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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