Summary of Study ST002249
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001437. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX31 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002249 |
Study Title | Sex-dependent effects of FGF21 on hepatic metabolism |
Study Summary | To investigate sex-dependent effects of fibroblast growth factor-21 (FGF21), diet-induced obese male and female wildtype mice were administered two acute doses of FGF21 or saline vehicle. Mice were euthanized, livers collected, and submitted for untargeted metabolomics analysis. |
Institute | University of California, Davis |
Department | Neurobiology, Physiology and Behavior |
Laboratory | Ryan |
Last Name | Chaffin |
First Name | Aki |
Address | 1 Shields Ave |
atchaffin@ucdavis.edu | |
Phone | 603-359-1907 |
Submit Date | 2022-08-09 |
Num Groups | 4 |
Total Subjects | 41 |
Num Males | 22 |
Num Females | 19 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2022-08-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001437 |
Project DOI: | doi: 10.21228/M8ZX31 |
Project Title: | Sex-dependent effects of FGF21 on hepatic metabolism |
Project Summary: | Untargeted metabolomics analysis of livers from male and female mice administered FGF21 or saline vehicle. |
Institute: | University of California, Davis |
Last Name: | Chaffin |
First Name: | Aki |
Address: | 1 Shields Ave |
Email: | atchaffin@ucdavis.edu |
Phone: | 603-359-1907 |
Publications: | https://doi.org/10.1172/jci.insight.155848 |
Subject:
Subject ID: | SU002335 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Gender: | Male and female |
Animal Light Cycle: | 12:12 |
Animal Feed: | 60% high-fat diet (Research Diets Cat# D12492) |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Sex | Treatment |
---|---|---|---|
SA216222 | 34_028 | Female | FGF21 |
SA216223 | 30_020 | Female | FGF21 |
SA216224 | 27_004 | Female | FGF21 |
SA216225 | 37_036 | Female | FGF21 |
SA216226 | 38_012 | Female | FGF21 |
SA216227 | 41_032 | Female | FGF21 |
SA216228 | 40_008 | Female | FGF21 |
SA216229 | 23_016 | Female | FGF21 |
SA216230 | 28_024 | Female | FGF21 |
SA216231 | 31_003 | Female | Saline |
SA216232 | 29_011 | Female | Saline |
SA216233 | 33_015 | Female | Saline |
SA216234 | 35_031 | Female | Saline |
SA216235 | 39_007 | Female | Saline |
SA216236 | 36_027 | Female | Saline |
SA216237 | 26_019 | Female | Saline |
SA216238 | 32_039 | Female | Saline |
SA216239 | 25_035 | Female | Saline |
SA216240 | 24_023 | Female | Saline |
SA216241 | 21_002 | Male | FGF21 |
SA216242 | 7_006 | Male | FGF21 |
SA216243 | 14_038 | Male | FGF21 |
SA216244 | 8_014 | Male | FGF21 |
SA216245 | 13_041 | Male | FGF21 |
SA216246 | 22_026 | Male | FGF21 |
SA216247 | 1_034 | Male | FGF21 |
SA216248 | 17_010 | Male | FGF21 |
SA216249 | 10_022 | Male | FGF21 |
SA216250 | 3_030 | Male | FGF21 |
SA216251 | 18_018 | Male | FGF21 |
SA216252 | 5_021 | Male | Saline |
SA216253 | 6_033 | Male | Saline |
SA216254 | 9_001 | Male | Saline |
SA216255 | 11_025 | Male | Saline |
SA216256 | 20_005 | Male | Saline |
SA216257 | 19_040 | Male | Saline |
SA216258 | 16_029 | Male | Saline |
SA216259 | 15_009 | Male | Saline |
SA216260 | 2_037 | Male | Saline |
SA216261 | 4_017 | Male | Saline |
SA216262 | 12_013 | Male | Saline |
Showing results 1 to 41 of 41 |
Collection:
Collection ID: | CO002328 |
Collection Summary: | Mice were euthanized and livers collected and frozen in liquid N2. |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002347 |
Treatment Summary: | Male and female C57BL/6J mice were placed on 60% high-fat diet (Research Diets, Cat# D12492) for approx. 10 weeks beginning at 10 weeks of age. Mice were then counterbalanced into two treatment groups based on body weight (n = 9-11 mice/ sex/ treatment). For experiment, one dose of FGF21 (0.1 mg/kg, ip) or saline vehicle was given right before the onset of the dark cycle, then another dose (0.1 mg/kg, ip) was given the following morning after the onset of the light cycle. Mice were euthanized two hours following the AM injection, livers collected, and frozen in liquid N2. |
Treatment Compound: | recombinant human FGF21 (ProSpecBio, Cat# CYT-474) |
Treatment Route: | intraperitoneal injection |
Treatment Dose: | 0.1 mg/kg |
Treatment Dosevolume: | 0.01 mg/mL |
Treatment Vehicle: | saline |
Sample Preparation:
Sampleprep ID: | SP002341 |
Sampleprep Summary: | See attached file. |
Sampleprep Protocol ID: | methods_sampleprep.pdf |
Sampleprep Protocol Filename: | methods_sampleprep.pdf |
Combined analysis:
Analysis ID | AN003675 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7890A |
Ion Mode | POSITIVE |
Units | peak |
Chromatography:
Chromatography ID: | CH002725 |
Methods Filename: | methods_GC.pdf |
Instrument Name: | Agilent 7890A |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS003426 |
Analysis ID: | AN003675 |
Instrument Name: | Agilent 7890A |
Instrument Type: | GC-TOF |
MS Type: | EI |
MS Comments: | See attached file. |
Ion Mode: | POSITIVE |
Analysis Protocol File: | methods_dataprocessing.pdf |