Summary of Study ST002313

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001483. The data can be accessed directly via it's Project DOI: 10.21228/M81M72 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002313
Study TitleDietary inclusion of nitrite-containing frankfurter exacerbates colorectal cancer pathology, increases oxidative stress, alters metabolism and causes gut dybiosis in APCmin mice
Study SummaryColorectal cancer (CRC) is the second most prevelant malignancy in Europe and diet is an important modifiable risk factor. Processed meat consumption, including meats with preservative salts such as sodium nitrite, have been implicated in CRC pathogenesis. This study investigated how the CRC pathology and metabolic status of adenomatous polyposis coli (APC) multiple intestinal neoplasia (min) mice was perturbed following 8 weeks of pork meat consumption.Dietary inclusions (15%) of either nitrite-free pork, nitrite-free sausage or nitrite-containing sausage (frankfurter) were compared against a parallel control group (100% chow). Comprehensive studies investigated: gastrointestinal tract histology (tumours, aberant crypt foci (ACF) and mucin deplin foci (MDF), lipid peroxidation (urine and serum), faecal microbiota and serum metabolomics (599 metabolites).
Institute
Institute for Global Food Security
Last NamePan
First NameXiaobei
Address19 Chlorine Gardens, Belfast, Antrim, BT9 5DL, United Kingdom
Emailx.pan@qub.ac.uk
Phone02890975984
Submit Date2022-09-20
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2022-11-03
Release Version1
Xiaobei Pan Xiaobei Pan
https://dx.doi.org/10.21228/M81M72
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001483
Project DOI:doi: 10.21228/M81M72
Project Title:Dietary inclusion of nitrite-containing frankfurter exacerbates colorectal cancer pathology, increases oxidative stress, alters metabolism and causes gut dybiosis in APCmin mice
Project Summary:This study investigated how the colorectal cancer pathology and metabolic status of adenomatous polyposis coli (APC) multiple intestinal neoplasia (min) mice was perturbed following 8 weeks of pork meat consumption.
Institute:Institute for Global Food Security
Last Name:Pan
First Name:Xiaobei
Address:19 Chlorine Gardens, Belfast, Antrim, BT9 5DL, United Kingdom
Email:x.pan@qub.ac.uk
Phone:02890975984

Subject:

Subject ID:SU002399
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Group Time point
SA227065M8TP1Control Week 0
SA227066M4TP1Control Week 0
SA227067M5TP1Control Week 0
SA227068M6TP1Control Week 0
SA227069M9TP1Control Week 0
SA227070M3TP1Control Week 0
SA227071M10TP1Control Week 0
SA227072M1TP1Control Week 0
SA227073M2TP1Control Week 0
SA227074M7TP1Control Week 0
SA227075M9TP2Control Week 8
SA227076M7TP2Control Week 8
SA227077M8TP2Control Week 8
SA227078M5TP2Control Week 8
SA227079M1TP2Control Week 8
SA227080M2TP2Control Week 8
SA227081M3TP2Control Week 8
SA227082M10TP2Control Week 8
SA227083M4TP2Control Week 8
SA227084M35TP1Frankfurter Week 0
SA227085M39TP1Frankfurter Week 0
SA227086M37TP1Frankfurter Week 0
SA227087M40TP1Frankfurter Week 0
SA227088M36TP1Frankfurter Week 0
SA227089M33TP1Frankfurter Week 0
SA227090M31TP1Frankfurter Week 0
SA227091M38TP1Frankfurter Week 0
SA227092M34TP1Frankfurter Week 0
SA227093M32TP1Frankfurter Week 0
SA227094M38TP2Frankfurter Week 8
SA227095M40TP2Frankfurter Week 8
SA227096M39TP2Frankfurter Week 8
SA227097M32TP2Frankfurter Week 8
SA227098M37TP2Frankfurter Week 8
SA227099M31TP2Frankfurter Week 8
SA227100M34TP2Frankfurter Week 8
SA227101M33TP2Frankfurter Week 8
SA227102M35TP2Frankfurter Week 8
SA227103M36TP2Frankfurter Week 8
SA227104M17TP1Pork Week 0
SA227105M16TP1Pork Week 0
SA227106M18TP1Pork Week 0
SA227107M15TP1Pork Week 0
SA227108M20TP1Pork Week 0
SA227109M19TP1Pork Week 0
SA227110M11TP1Pork Week 0
SA227111M12TP1Pork Week 0
SA227112M14TP1Pork Week 0
SA227113M13TP1Pork Week 0
SA227114M20TP2Pork Week 8
SA227115M11TP2Pork Week 8
SA227116M14TP2Pork Week 8
SA227117M12TP2Pork Week 8
SA227118M19TP2Pork Week 8
SA227119M18TP2Pork Week 8
SA227120M15TP2Pork Week 8
SA227121M13TP2Pork Week 8
SA227122M16TP2Pork Week 8
SA227123M17TP2Pork Week 8
SA227124M26TP1Sausage Week 0
SA227125M25TP1Sausage Week 0
SA227126M29TP1Sausage Week 0
SA227127M30TP1Sausage Week 0
SA227128M27TP1Sausage Week 0
SA227129M28TP1Sausage Week 0
SA227130M21TP1Sausage Week 0
SA227131M24TP1Sausage Week 0
SA227132M22TP1Sausage Week 0
SA227133M23TP1Sausage Week 0
SA227134M28TP2Sausage Week 8
SA227135M30TP2Sausage Week 8
SA227136M29TP2Sausage Week 8
SA227137M22TP2Sausage Week 8
SA227138M21TP2Sausage Week 8
SA227139M23TP2Sausage Week 8
SA227140M24TP2Sausage Week 8
SA227141M25TP2Sausage Week 8
SA227142M26TP2Sausage Week 8
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Collection:

Collection ID:CO002392
Collection Summary:Blood samples were collected from the tails of all animals at week 0 and a final cardiac puncture blood was taken at the termination of the study (week 8).
Sample Type:Blood (serum)
Storage Conditions:-20℃

Treatment:

Treatment ID:TR002411
Treatment Summary:Each treatment group received one of four diets for the duration of the study, the dietary groups included; Control, which consisted of 100% AIN76 (Altromin, Germany). Sausage meat (15%)/AIN76 (85%), Frankfurter (15%)/AIN76 (85%), unprocessed pork (15%)/AIN76 (85%).All meat was freeze dried by European Freeze Dry (UK) and powderized. Altromin then incorporated this into pellet composed of 15% meat powder and 85% AIN76.

Sample Preparation:

Sampleprep ID:SP002405
Sampleprep Summary:Samples were dispensed into a 96-well plate, dried at room temperature for 30 min and then 50 µL of phenylisothiocyanate (PITC) was added. After a further 20 min incubation at room temperature samples were dried under nitrogen for 1 h. Ammonium acetate (3 mM) was added, and the plate was shaken at room temperature for 30 min and centrifuged for 2 min at 500 g. Each well then had 150 µL transferred from it to a 96 deep well plate along with and equal volume of dH2O.

Combined analysis:

Analysis ID AN003779 AN003780
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity I-Class Waters Acquity I-Class
Column Biocrates Quant500 Biocrates Quant500
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Waters Xevo TQ-S Waters Xevo TQ-S
Ion Mode POSITIVE NEGATIVE
Units uM uM

Chromatography:

Chromatography ID:CH002794
Instrument Name:Waters Acquity I-Class
Column Name:Biocrates Quant500
Chromatography Type:Reversed phase

MS:

MS ID:MS003522
Analysis ID:AN003779
Instrument Name:Waters Xevo TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:ESI positive acquisition Data were acquired using Waters Masslynx and processed using the MetIDQ software.
Ion Mode:POSITIVE
  
MS ID:MS003523
Analysis ID:AN003780
Instrument Name:Waters Xevo TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:ESI negative acquisition Data were acquired using Waters Masslynxand processed using the MetIDQ software.
Ion Mode:NEGATIVE
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