Summary of Study ST002351

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001510. The data can be accessed directly via it's Project DOI: 10.21228/M8HD93 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002351
Study TitleCharacterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Atlanta ECHO Cohort)
Study TypeProspective Cohort Study
Study SummaryThe Atlanta ECHO Cohort is comprised of African American mother-child dyads who reside in the greater metropolitan Atlanta area. Enrollment begins during pregnancy from prenatal care clinics affiliated with two metro hospital systems. During pregnancy, data and biospecimens are obtained during an initial study visit (between 8-14 weeks gestation) and during a second study visit (between 24-30 weeks gestation). In the perinatal period, we collect the residual newborn blood spot (obtained from the neonate during the delivery hospitalization for state metabolic screening). Details of pregnancy complications, birth, and neonatal outcomes are ascertained via medical record abstraction. Children are then followed annually via in-person clinical assessments and maternal questionnaire data. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The Atlanta ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Emory University
DepartmentSchool of Medicine, Department of Gynecology & Obstetrics; School of Public Health, Department of Environmental Health
Last NameDunlop
First NameAnne
Address101 Woodruff Circle, Rm 4303 Woodruff Memorial Building, Atlanta, GA 30322
Emailamlang@emory.edu
Phone(404) 712-8520
Submit Date2022-11-14
Total Subjects564
Study CommentsHHEAR Project EM19-0009, ECHO Project EC0374
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-11-14
Release Version1
Anne Dunlop Anne Dunlop
https://dx.doi.org/10.21228/M8HD93
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001510
Project DOI:doi: 10.21228/M8HD93
Project Title:Characterizing the intrauterine environment via untargeted metabolomics profiling of maternal blood collected during pregnancy (Atlanta ECHO Cohort)
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) program, and uses data from five cohorts: Healthy Start, Project Viva, Atlanta ECHO, Paneth Cohort, and PETALS. This project will generate new untargeted metabolomics profiling data at two time-points in pregnancy for all women in order to identify trimester-specific metabolomics signatures and longitudinal metabolomics trajectories associated with offspring birth size; identify the contribution of maternal sociodemographic and perinatal characteristics to the metabolomics signatures.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:9843770693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The Atlanta ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539

Subject:

Subject ID:SU002440
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA235768PZDu_HHEAR_39HHEAR Pool
SA235769PZDu_HHEAR_38HHEAR Pool
SA235770PZDu_HHEAR_40HHEAR Pool
SA235771PZDu_HHEAR_42HHEAR Pool
SA235772PZDu_HHEAR_43HHEAR Pool
SA235773PZDu_HHEAR_37HHEAR Pool
SA235774PZDu_HHEAR_41HHEAR Pool
SA235775PZDu_HHEAR_36HHEAR Pool
SA235776PZDu_HHEAR_32HHEAR Pool
SA235777PZDu_HHEAR_31HHEAR Pool
SA235778PZDu_HHEAR_33HHEAR Pool
SA235779PZDu_HHEAR_34HHEAR Pool
SA235780PZDu_HHEAR_35HHEAR Pool
SA235781PZDu_HHEAR_44HHEAR Pool
SA235782PZDu_HHEAR_45HHEAR Pool
SA235783PZDu_HHEAR_55HHEAR Pool
SA235784PZDu_HHEAR_53HHEAR Pool
SA235785PZDu_HHEAR_56HHEAR Pool
SA235786PZDu_HHEAR_57HHEAR Pool
SA235787PZDu_HHEAR_1HHEAR Pool
SA235788PZDu_HHEAR_52HHEAR Pool
SA235789PZDu_HHEAR_51HHEAR Pool
SA235790PZDu_HHEAR_47HHEAR Pool
SA235791PZDu_HHEAR_46HHEAR Pool
SA235792PZDu_HHEAR_48HHEAR Pool
SA235793PZDu_HHEAR_49HHEAR Pool
SA235794PZDu_HHEAR_50HHEAR Pool
SA235795PZDu_HHEAR_30HHEAR Pool
SA235796PZDu_HHEAR_54HHEAR Pool
SA235797PZDu_HHEAR_10HHEAR Pool
SA235798PZDu_HHEAR_9HHEAR Pool
SA235799PZDu_HHEAR_29HHEAR Pool
SA235800PZDu_HHEAR_12HHEAR Pool
SA235801PZDu_HHEAR_14HHEAR Pool
SA235802PZDu_HHEAR_13HHEAR Pool
SA235803PZDu_HHEAR_8HHEAR Pool
SA235804PZDu_HHEAR_7HHEAR Pool
SA235805PZDu_HHEAR_4HHEAR Pool
SA235806PZDu_HHEAR_3HHEAR Pool
SA235807PZDu_HHEAR_5HHEAR Pool
SA235808PZDu_HHEAR_6HHEAR Pool
SA235809PZDu_HHEAR_2HHEAR Pool
SA235810PZDu_HHEAR_15HHEAR Pool
SA235811PZDu_HHEAR_11HHEAR Pool
SA235812PZDu_HHEAR_25HHEAR Pool
SA235813PZDu_HHEAR_24HHEAR Pool
SA235814PZDu_HHEAR_16HHEAR Pool
SA235815PZDu_HHEAR_27HHEAR Pool
SA235816PZDu_HHEAR_28HHEAR Pool
SA235817PZDu_HHEAR_23HHEAR Pool
SA235818PZDu_HHEAR_26HHEAR Pool
SA235819PZDu_HHEAR_17HHEAR Pool
SA235820PZDu_HHEAR_22HHEAR Pool
SA235821PZDu_HHEAR_19HHEAR Pool
SA235822PZDu_HHEAR_18HHEAR Pool
SA235823PZDu_HHEAR_21HHEAR Pool
SA235824PZDu_HHEAR_20HHEAR Pool
SA235825PZDu_NIST_14NIST Pool
SA235826PZDu_NIST_15NIST Pool
SA235827PZDu_NIST_13NIST Pool
SA235828PZDu_NIST_16NIST Pool
SA235829PZDu_NIST_20NIST Pool
SA235830PZDu_NIST_21NIST Pool
SA235831PZDu_NIST_19NIST Pool
SA235832PZDu_NIST_18NIST Pool
SA235833PZDu_NIST_17NIST Pool
SA235834PZDu_NIST_12NIST Pool
SA235835PZDu_NIST_4NIST Pool
SA235836PZDu_NIST_2NIST Pool
SA235837PZDu_NIST_1NIST Pool
SA235838PZDu_NIST_11NIST Pool
SA235839PZDu_NIST_5NIST Pool
SA235840PZDu_NIST_3NIST Pool
SA235841PZDu_NIST_6NIST Pool
SA235842PZDu_NIST_9NIST Pool
SA235843PZDu_NIST_8NIST Pool
SA235844PZDu_NIST_7NIST Pool
SA235845PZDu_NIST_10NIST Pool
SA235846PZDu_SP_20Study Pool
SA235847PZDu_SP_19Study Pool
SA235848PZDu_SP_16Study Pool
SA235849PZDu_SP_21Study Pool
SA235850PZDu_SP_17Study Pool
SA235851PZDu_SP_18Study Pool
SA235852PZDu_SP_27Study Pool
SA235853PZDu_SP_15Study Pool
SA235854PZDu_SP_28Study Pool
SA235855PZDu_SP_26Study Pool
SA235856PZDu_SP_25Study Pool
SA235857PZDu_SP_23Study Pool
SA235858PZDu_SP_24Study Pool
SA235859PZDu_SP_22Study Pool
SA235860PZDu_SP_9Study Pool
SA235861PZDu_SP_3Study Pool
SA235862PZDu_SP_4Study Pool
SA235863PZDu_SP_2Study Pool
SA235864PZDu_SP_1Study Pool
SA235865PZDu_SP_29Study Pool
SA235866PZDu_SP_5Study Pool
SA235867PZDu_SP_7Study Pool
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Collection:

Collection ID:CO002433
Collection Summary:The Atlanta ECHO Cohort is comprised of African American mother-child dyads who reside in the greater metropolitan Atlanta area. Enrollment begins during pregnancy from prenatal care clinics affiliated with two metro hospital systems. During pregnancy, data and biospecimens are obtained during an initial study visit (between 8-14 weeks gestation) and during a second study visit (between 24-30 weeks gestation). In the perinatal period, we collect the residual newborn blood spot (obtained from the neonate during the delivery hospitalization for state metabolic screening). Details of pregnancy complications, birth, and neonatal outcomes are ascertained via medical record abstraction. Children are then followed annually via in-person clinical assessments and maternal questionnaire data.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002452
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002446
Sampleprep Summary:Serum samples (100 µL per tube) were shipped from Emory University to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the study samples to a new set of tubes and used them for the analysis. An additional 10 µL was taken from the original study sample and transferred to another tube to make the total study pool for this project, and then distributed with 50 µL per aliquot, used as Quality control study pools (QC study pools) throughout the whole analysis. All sample aliquots (50 µL each) and QC study pools (50 µL each) were stored at -80° C until the day for sample preparation. HHEAR reference plasma (50 µL each) and NIST serum (909c) reference material (50 µL each) were provided by the NC HHEAR Hub. The LC-MS grade water (50 µL) was used as blank. Sample preparation was conducted by batch, and all samples (except blank) were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference serum, and blanks, were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extract Storage:Described in summary

Combined analysis:

Analysis ID AN003837
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Scientific™ Vanquish™ UPHPLC
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Normalized intensity

Chromatography:

Chromatography ID:CH002841
Instrument Name:Thermo Scientific™ Vanquish™ UPHPLC
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000 psi
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003579
Analysis ID:AN003837
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 KV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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