Summary of Study ST002441

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001572. The data can be accessed directly via it's Project DOI: 10.21228/M8H70Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002441
Study TitleImpacts of interactions between environmental chemical exposures and diet on gut microbiota and microbiota-derived metabolites in mothers and children (MARCH Cohort)
Study TypeProspective Cohort Study
Study SummaryMARCH is a prospective population based pregnancy cohort that recruited pregnant participants from 2017-2023 from 11 sites within Michigan. Women over 18 years of age were recruited at first prenatal visit and had 3 data collection points focused around each trimester of pregnancy. Blood, urine, and questionnaire data were collected at each prenatal visit. At birth placenta was collected and after birth, infants were assessed at 3 months, 9 months, and yearly. For more information on MARCH please contact Jean Kerver; kerverje@msu.edu This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. MARCH is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539
Institute
Wayne State University
DepartmentPharmacology
Last NamePetriello
First NameMichael
Address6135 Woodward Ave, Detroit, MI 48202
Emailmichael.petriello@wayne.edu
Phone313-577-1089
Submit Date2023-01-12
Total Subjects65
Study CommentsECHO Project EC0549, HHEAR Project E020-0012
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-01-12
Release Version1
Michael Petriello Michael Petriello
https://dx.doi.org/10.21228/M8H70Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001572
Project DOI:doi: 10.21228/M8H70Z
Project Title:Impacts of interactions between environmental chemical exposures and diet on gut microbiota and microbiota-derived metabolites in mothers and children
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:This project is funded by the NIH Environmental influences on Child Health Outcomes (ECHO) OIF program, and uses data from two cohorts: MAAP and MARCH. This project will examine associations with PFAS exposures and gut microbiota form and function in mothers and children over time. Untargeted metabolomics will be used as a means to assess changes in microbiota-derived metabolites.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuanyuan
Address:Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:984-377-0693
Funding Source:This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. MAAP is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; (U24 ES026539)

Subject:

Subject ID:SU002530
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA244107MARCH_HHEAR_6HHEAR Pool
SA244108MARCH_HHEAR_1HHEAR Pool
SA244109MARCH_HHEAR_5HHEAR Pool
SA244110MARCH_HHEAR_7HHEAR Pool
SA244111MARCH_HHEAR_3HHEAR Pool
SA244112MARCH_HHEAR_2HHEAR Pool
SA244113MARCH_HHEAR_4HHEAR Pool
SA244114MARCH_NIST_3NIST Pool
SA244115MARCH_NIST_2NIST Pool
SA244116MARCH_NIST_1NIST Pool
SA244117MARCH_SP_2Study Pool
SA244118MARCH_SP_4Study Pool
SA244119MARCH_SP_1Study Pool
SA244120MARCH_SP_3Study Pool
SA244121MARCH_SP_6Study Pool
SA244122MARCH_SP_7Study Pool
SA244123MARCH_SP_5Study Pool
SA244124MARCH_S_46Study Sample
SA244125MARCH_S_47Study Sample
SA244126MARCH_S_48Study Sample
SA244127MARCH_S_50Study Sample
SA244128MARCH_S_49Study Sample
SA244129MARCH_S_45Study Sample
SA244130MARCH_S_43Study Sample
SA244131MARCH_S_40Study Sample
SA244132MARCH_S_39Study Sample
SA244133MARCH_S_41Study Sample
SA244134MARCH_S_42Study Sample
SA244135MARCH_S_51Study Sample
SA244136MARCH_S_44Study Sample
SA244137MARCH_S_61Study Sample
SA244138MARCH_S_38Study Sample
SA244139MARCH_S_60Study Sample
SA244140MARCH_S_62Study Sample
SA244141MARCH_S_63Study Sample
SA244142MARCH_S_65Study Sample
SA244143MARCH_S_64Study Sample
SA244144MARCH_S_59Study Sample
SA244145MARCH_S_58Study Sample
SA244146MARCH_S_54Study Sample
SA244147MARCH_S_53Study Sample
SA244148MARCH_S_55Study Sample
SA244149MARCH_S_56Study Sample
SA244150MARCH_S_57Study Sample
SA244151MARCH_S_52Study Sample
SA244152MARCH_S_32Study Sample
SA244153MARCH_S_12Study Sample
SA244154MARCH_S_11Study Sample
SA244155MARCH_S_10Study Sample
SA244156MARCH_S_13Study Sample
SA244157MARCH_S_14Study Sample
SA244158MARCH_S_17Study Sample
SA244159MARCH_S_16Study Sample
SA244160MARCH_S_15Study Sample
SA244161MARCH_S_9Study Sample
SA244162MARCH_S_8Study Sample
SA244163MARCH_S_3Study Sample
SA244164MARCH_S_2Study Sample
SA244165MARCH_S_1Study Sample
SA244166MARCH_S_4Study Sample
SA244167MARCH_S_5Study Sample
SA244168MARCH_S_7Study Sample
SA244169MARCH_S_6Study Sample
SA244170MARCH_S_18Study Sample
SA244171MARCH_S_19Study Sample
SA244172MARCH_S_31Study Sample
SA244173MARCH_S_30Study Sample
SA244174MARCH_S_29Study Sample
SA244175MARCH_S_33Study Sample
SA244176MARCH_S_34Study Sample
SA244177MARCH_S_36Study Sample
SA244178MARCH_S_35Study Sample
SA244179MARCH_S_28Study Sample
SA244180MARCH_S_27Study Sample
SA244181MARCH_S_22Study Sample
SA244182MARCH_S_21Study Sample
SA244183MARCH_S_20Study Sample
SA244184MARCH_S_23Study Sample
SA244185MARCH_S_24Study Sample
SA244186MARCH_S_26Study Sample
SA244187MARCH_S_25Study Sample
SA244188MARCH_S_37Study Sample
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Collection:

Collection ID:CO002523
Collection Summary:MARCH is a prospective population based pregnancy cohort that recruited pregnant participants from 2017-2023 from 11 sites within Michigan. Women over 18 years of age were recruited at first prenatal visit and had 3 data collection points focused around each trimester of pregnancy. Blood, urine, and questionnaire data were collected at each prenatal visit. At birth placenta was collected and after birth, infants were assessed at 3 months, 9 months, and yearly. For more information on MARCH please contact Jean Kerver; kerverje@msu.edu
Sample Type:Blood (plasma)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002542
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002536
Sampleprep Summary:Study samples (500 µL aliquots) were shipped from Wayne State University to the NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and aliquoted the study samples at 50 µL per aliquot. An additional 10 µL was taken from each study sample, mixed, and distributed at 50 µL per aliquot, which were used as QC study pools throughout the study. HHEAR reference plasma (50 µL each) and NIST plasma (1950) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blank. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference plasma, NIST reference plasma, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN003978
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH002941
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000 psi
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 mL/min
Injection Temperature:8
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:10:90 Methanol:Water with 0.1% FA solution
Strong Wash Solvent Name:75:25 2-Propanol:Water with 0.1% FA solution
Chromatography Type:Reversed phase

MS:

MS ID:MS003712
Analysis ID:AN003978
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.4 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Capillary Voltage:3.5 kV
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Temp:400°C
Fragmentation Method:CID
Desolvation Gas Flow:55
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