Summary of Study ST002491

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001608. The data can be accessed directly via it's Project DOI: 10.21228/M8VB03 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002491
Study TitleSix tryptophan metabolites in mouse serum
Study SummaryWe establish that six tryptophan metabolites are essentially not CYP1A1 substrates and the level of these metabolite in mouse serum are not affected by a lack of CYP1A1 or any other Aryl hydrocarbon Receptor (AHR) dependent metabolic pathway expression in vivo. These results establish that tryptophan metabolites that circulate at significant levels in vivo are not subject to an autoregulatory feedback loop between the AHR and CYP1A1.
Institute
Pennsylvania State University
Last NameDong
First NameFancong
Address309 Life Sciences Building, University Park, PA 16802
Emailfxd93@psu.edu
Phone8148651415
Submit Date2023-02-22
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2023-04-03
Release Version1
Fancong Dong Fancong Dong
https://dx.doi.org/10.21228/M8VB03
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001608
Project DOI:doi: 10.21228/M8VB03
Project Title:Tryptophan metabolites that circulate at significant levels in vivo are not subject to an autoregulatory feedback loop between the AHR and CYP1A1.
Project Type:MS quantitative study
Project Summary:We establish that six tryptophan metabolites are essentially not CYP1A1 substrates and the level of these metabolite in mouse serum are not affected by a lack of CYP1A1 or any other Aryl hydrocarbon Receptor (AHR) dependent metabolic pathway expression in vivo. These results establish that tryptophan metabolites that circulate at significant levels in vivo are not subject to an autoregulatory feedback loop between the AHR and CYP1A1.
Institute:The Pennsylvania State University
Last Name:Dong
First Name:Fancong
Address:309 Life Sciences Building, State college, PA, 16802, USA
Email:fxd93@psu.edu
Phone:8148651415

Subject:

Subject ID:SU002581
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA2489061_STD8-
SA2489071_STD9-
SA2489081_STD10-
SA2489091_STD7-
SA2489101_STD4-
SA2489111_STD2-
SA2489121_STD3-
SA2489132_STD1-
SA2489141_STD6-
SA2489152_STD3-
SA2489162_STD8-
SA2489172_STD9-
SA2489182_STD10-
SA2489192_STD7-
SA2489202_STD6-
SA2489211_STD1-
SA2489222_STD4-
SA2489232_STD5-
SA2489242_STD2-
SA2489251_STD5-
SA2489261_SERUM_WT4AHR+/+
SA2489271_SERUM_WT5AHR+/+
SA2489281_SERUM_WT3AHR+/+
SA2489291_SERUM_WT1AHR+/+
SA2489301_SERUM_WT10AHR+/+
SA2489311_SERUM_WT6AHR+/+
SA2489321_SERUM_WT2AHR+/+
SA2489331_SERUM_WT11AHR+/+
SA2489341_SERUM_WT12AHR+/+
SA2489351_SERUM_WT9AHR+/+
SA2489361_SERUM_WT7AHR+/+
SA2489371_SERUM_WT8AHR+/+
SA2489381_SERUM_HETS5AHR+/-
SA2489391_SERUM_HETS6AHR+/-
SA2489401_SERUM_HETS1AHR+/-
SA2489411_SERUM_HETS4AHR+/-
SA2489421_SERUM_HETS3AHR+/-
SA2489431_SERUM_HETS2AHR+/-
SA2489441_SERUM_KO4AHR-/-
SA2489451_SERUM_KO5AHR-/-
SA2489461_SERUM_KO3AHR-/-
SA2489471_SERUM_KO1AHR-/-
SA2489481_SERUM_KO6AHR-/-
SA2489491_SERUM_KO2AHR-/-
SA2489502_SERUM_C5wild type
SA2489512_SERUM_C4wild type
SA2489522_SERUM_C3wild type
SA2489532_SERUM_C2wild type
SA2489542_SERUM_C1wild type
SA2489552_SERUM_C6wild type
SA2489562_SERUM_PCB1wild type
SA2489572_SERUM_PCB5wild type
SA2489582_SERUM_PCB4wild type
SA2489592_SERUM_PCB3wild type
SA2489602_SERUM_PCB2wild type
SA2489612_SERUM_PCB6wild type
Showing results 1 to 56 of 56

Collection:

Collection ID:CO002574
Collection Summary:Serum samples from mice were collected and stored at -80°C.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR002593
Treatment Summary:Thawed on ice, serum sample was mixed with 4 volume extraction solvent of ice-cold methanol containing indole-3-acetic acid-d4 and kynurenic acid-d5. Mixture was vortexed and incubated at −20 °C for 30 min. Following centrifugation at 12,000 × g for 15 min at 4℃, supernatant was collected and subsequently evaporated to dryness (Thermo Scientific, Waltham, MA) and dissolved in 10% acetonitrile containing 1 µM chlorpropamide. After centrifugation at 12,000 × g for 15 min at 4℃, supernatants were transferred to autosampler vials for LC-MS analysis.

Sample Preparation:

Sampleprep ID:SP002587
Sampleprep Summary:C57BL6/J mice were obtained from Jackson Laboratory (Bar Harbor, ME), bred in-house and maintained on a chow diet. Ahr+/-, and Ahr-/- mice were kindly provided by Dr. Christopher Bradfield (University of Wisconsin-Madison) and bred using female Ahr+/- and male Ahr-/- mice. Mice were housed on corncob bedding in a temperature- and light-controlled facility and given access to food and water ad libitum. Mice were also maintained in a pathogen-free facility and treated humanely with approval from the Animal Care and Use Committee of the Pennsylvania State University and methods were carried out in accordance with approved guidelines. Mice in the 8-10-week-old range were utilized in all experiments. Serum samples obtained from mice exposed to PCB126 for 5 days was previously described. Briefly, mice were fed dough pills once every 24 h, each pill contained 24 µg/kg PCB126 for five days and mice were sacrificed on day 6.

Combined analysis:

Analysis ID AN004065
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu 20AD
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple TOF
MS instrument name ABI Sciex 5600 TripleTOF
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH003011
Instrument Name:Shimadzu 20AD
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:55
Flow Gradient:The initial condition were 97% A and 3 % B, increasing to 45% B at 10 min, 75% B at 12 min where it was held at 75% B until 17.5 min before returning to the initial conditions.
Flow Rate:0.25 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003812
Analysis ID:AN004065
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:Peakview was used for data analysis.
Ion Mode:POSITIVE
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