Summary of Study ST003095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003095 |
| Study Title | Lactiplantibacillus plantarum intervention on serum choline derivatives of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of serum choline derivatives of germ-free mice. L. plantarum during gestation of humanized GF mice showed no significant differences on the serum choline derivatives. |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai distric |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA333311 | P220801156 | serum | control |
| SA333312 | P220801159 | serum | control |
| SA333313 | P220801157 | serum | control |
| SA333314 | P220801158 | serum | control |
| SA333315 | P220801165 | serum | intervention |
| SA333316 | P220801164 | serum | intervention |
| SA333317 | P220801161 | serum | intervention |
| SA333318 | P220801160 | serum | intervention |
| SA333319 | P220801162 | serum | intervention |
| SA333320 | P220801163 | serum | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003203 |
| Collection Summary: | Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003219 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003216 |
| Sampleprep Summary: | Samples were added in a 2 mL centrifuge tube, add 10 μL of internal standard solution, then add 750 μL of 1% formic acid-acetonitrile solution, vortex, centrifuge for 10 min at 12,000 rpm at 4^oC, and then resovle and filter the supernatant by 0.22 μm membrane for LC-MS analysis. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005066 |
|---|---|
| Analysis type | MS |
| Chromatography type | LC |
| Chromatography system | AB SCIEX Jasper HPLC |
| Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE |
| Units | μmol/L |
Chromatography:
| Chromatography ID: | CH003825 |
| Instrument Name: | AB SCIEX Jasper HPLC |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~1 min, 80% B; 1~2 min, 80~70% B; 2~2.5 min, 70% B; 2.5~3 min, 70~50% B; 3~3.5 min, 50% B; 3.5~4 min, 50~80% B; 4~6 min, 80% B |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004804 |
| Analysis ID: | AN005066 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. Multiple reaction monitoring (MRM) was employed. The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | POSITIVE |
