Summary of Study ST003096
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003096 |
| Study Title | Lactiplantibacillus plantarum intervention on cecum short-chain fatty acids of gestational fecal microbiome transplant germ-free mice |
| Study Summary | The study aims to investigate whether administration of probiotic L. plantarum could influence profiling of cecum short-chain fatty acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that caproic acid was significantly increased. Other SCFAs (acetic acid, propionic acid, isovaleric acid, valeric acid, and isobutyric acid) showed no significant difference |
| Institute | Beijing Sanyuan Foods Co. Ltd |
| Last Name | Lijun |
| First Name | Chen |
| Address | 8th yingchang street, yinghai distric |
| chenlijun@sanyuan.com.cn | |
| Phone | 01056306594 |
| Submit Date | 2024-02-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001923 |
| Project DOI: | doi: 10.21228/M85149 |
| Project Title: | Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice |
| Project Summary: | The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism. |
| Institute: | Beijing Sanyuan Foods Co. Ltd |
| Last Name: | Lijun |
| First Name: | Chen |
| Address: | 8th yingchang street, yinghai distric |
| Email: | chenlijun@sanyuan.com.cn |
| Phone: | 01056306594 |
Subject:
| Subject ID: | SU003211 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Factor |
|---|---|---|---|
| SA333321 | 81 | cecum content | control |
| SA333322 | 122 | cecum content | control |
| SA333323 | 107 | cecum content | control |
| SA333324 | 120 | cecum content | control |
| SA333325 | 909 | cecum content | intervention |
| SA333326 | 908 | cecum content | intervention |
| SA333327 | 185 | cecum content | intervention |
| SA333328 | 180 | cecum content | intervention |
| SA333329 | 188 | cecum content | intervention |
| SA333330 | 907 | cecum content | intervention |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO003204 |
| Collection Summary: | Cecum content was collected and stored at -80°C environment |
| Sample Type: | Cecum |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003220 |
| Treatment Summary: | mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003217 |
| Sampleprep Summary: | 100 mg of sample was extracted in 50 μL of 15% phosphoric acid with 100 μL of 125 μg/mL 4-methyl valeric acid solution as internal standard and 400 μL ether, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 1 min and the supernatant was transferred into the vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005067 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Trace 1310 |
| Column | Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo ISQ LT |
| Ion Mode | UNSPECIFIED |
| Units | μg/g |
Chromatography:
| Chromatography ID: | CH003826 |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm) |
| Column Temperature: | The column temperature was programmed to increase from an initial temperature of 90°C, followed by an increase to 120 °C at 10 °C/min, and to 150 °C at 5 °C/min, and finally to 250 °C at 25 °C/min which was maintained for 2 min |
| Flow Gradient: | none |
| Flow Rate: | 1 mL/min |
| Solvent A: | none |
| Solvent B: | none |
| Chromatography Type: | GC |
MS:
| MS ID: | MS004805 |
| Analysis ID: | AN005067 |
| Instrument Name: | Thermo ISQ LT |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | Single ion monitoring (SIM) mode was used with the electron energy of 70 eV. MS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” format raw data to “.mzML” format, which would be used for downstream analysis. |
| Ion Mode: | UNSPECIFIED |
