Summary of Study ST003097

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003097
Study TitleLactiplantibacillus plantarum intervention on cecum bile acids of gestational fecal microbiome transplant germ-free mice
Study SummaryThe study aims to investigate whether administration of probiotic L. plantarum could influence profiling of cecum bile acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that allolithocholic acid was significantly increased. Other bile acids showed no significant difference.
Institute
Beijing Sanyuan Foods Co. Ltd
Last NameLijun
First NameChen
Address8th yingchang street, yinghai district
Emailchenlijun@sanyuan.com.cn
Phone01056306594
Submit Date2024-02-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-03-08
Release Version1
Chen Lijun Chen Lijun
https://dx.doi.org/10.21228/M85149
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001923
Project DOI:doi: 10.21228/M85149
Project Title:Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice
Project Summary:The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism.
Institute:Beijing Sanyuan Foods Co. Ltd
Last Name:Lijun
First Name:Chen
Address:8th yingchang street, yinghai distric
Email:chenlijun@sanyuan.com.cn
Phone:01056306594

Subject:

Subject ID:SU003212
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA333331908MNcecum content control
SA333332185MNcecum content control
SA333333188MNcecum content control
SA333334909MNcecum content control
SA333335122MNcecum content intervention
SA333336120MNcecum content intervention
SA333337907MNcecum content intervention
SA333338180MNcecum content intervention
SA33333981MNcecum content intervention
SA333340107MNcecum content intervention
Showing results 1 to 10 of 10

Collection:

Collection ID:CO003205
Collection Summary:Cecum content sample was collected and stored at -80°C environment for bile acids analysis.
Sample Type:Cecum
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003221
Treatment Summary:Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).

Sample Preparation:

Sampleprep ID:SP003218
Sampleprep Summary:Sample was extracted in 400 μL of methanol, and vortexing and shaking for 60 s, add 100 mg of glass beads, and put into a high-throughput tissue grinder, grinding at 55 Hz for 60 s. Repeat the above operation at least twice; ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL of 30% methanol solution, and add it to the assay vial.
Processing Storage Conditions:4℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN005068
Analysis type MS
Chromatography type LC
Chromatography system Shimmadzu LC-30
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Single quadrupole
MS instrument name ABI Sciex 6500+
Ion Mode NEGATIVE
Units μg/g

Chromatography:

Chromatography ID:CH003827
Instrument Name:Shimmadzu LC-30
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:0~9 min, 30% B; 9~14 min, 30~36% B;14~18 min, 36~38% B;18~24 min, 38~50% B;24~32 min, 50~75% B;32~33 min, 75~90% B;33~35.5 min, 90~30% B
Flow Rate:5 μL/min
Solvent A:0.01% formic acid in water
Solvent B:100% acetonitrile
Chromatography Type:LC

MS:

MS ID:MS004806
Analysis ID:AN005068
Instrument Name:ABI Sciex 6500+
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:Ion source temperature 500°C, ion source voltage -4500 V, collision gas 6 psi, air curtain gas 30 psi, nebulizer gas and auxiliary gas both 50 psi. Scanning was performed using multiple reaction monitoring (MRM). The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:NEGATIVE
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