Summary of Study ST003100

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001923. The data can be accessed directly via it's Project DOI: 10.21228/M85149 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003100
Study TitleLactiplantibacillus plantarum intervention on fecal bile acids of gestational fecal microbiome transplant germ-free mice
Study SummaryThe study aims to investigate whether administration of probiotic L. plantarum could influence profiling of fecal bile acids of germ-free mice. L. plantarum during gestation of humanized GF mice showed that α-MCA, UCA, ACA, β-UDCA, and isoLCA were significantly increased. Other bile acids showed no significant difference.
Institute
Beijing Sanyuan Foods Co. Ltd
Last NameLijun
First NameChen
Address8th yingchang street, yinghai district
Emailchenlijun@sanyuan.com.cn
Phone01056306594
Submit Date2024-02-18
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-03-08
Release Version1
Chen Lijun Chen Lijun
https://dx.doi.org/10.21228/M85149
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001923
Project DOI:doi: 10.21228/M85149
Project Title:Lactiplantibacillus plantarum intervention on gestational health of gestational fecal microbiome transplant germ-free mice
Project Summary:The study aims to investigate whether administration of probiotic L. plantarum could influence comprehensive gestational health by using germ-free mice, and characterizing its influence on relationships between the gut microbiome composition and metabolism.
Institute:Beijing Sanyuan Foods Co. Ltd
Last Name:Lijun
First Name:Chen
Address:8th yingchang street, yinghai distric
Email:chenlijun@sanyuan.com.cn
Phone:01056306594

Subject:

Subject ID:SU003215
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA333401188S14fecal control
SA333402188S18fecal control
SA333403188S7fecal control
SA333404188S1fecal control
SA333405185S7fecal control
SA333406185S14fecal control
SA333407185S18fecal control
SA333408908S0fecal control
SA333409188S0fecal control
SA333410908S14fecal control
SA333411909S14fecal control
SA333412909S18fecal control
SA333413909S7fecal control
SA333414909S1fecal control
SA333415909S0fecal control
SA333416185S1fecal control
SA333417908S18fecal control
SA333418908S7fecal control
SA333419908S1fecal control
SA333420185S0fecal control
SA333421120S0fecal intervention
SA333422107S7fecal intervention
SA333423120S1fecal intervention
SA333424907S7fecal intervention
SA333425907S14fecal intervention
SA333426907S18fecal intervention
SA333427107S18fecal intervention
SA333428107S14fecal intervention
SA333429081S14fecal intervention
SA333430081S1fecal intervention
SA333431081S18fecal intervention
SA333432081S7fecal intervention
SA333433107S1fecal intervention
SA333434107S0fecal intervention
SA333435907S1fecal intervention
SA333436907S0fecal intervention
SA333437180S0fecal intervention
SA333438122S7fecal intervention
SA333439081S0fecal intervention
SA333440180S7fecal intervention
SA333441180S14fecal intervention
SA333442180S18fecal intervention
SA333443122S18fecal intervention
SA333444122S14fecal intervention
SA333445120S18fecal intervention
SA333446120S14fecal intervention
SA333447120S7fecal intervention
SA333448122S0fecal intervention
SA333449122S1fecal intervention
SA333450180S1fecal intervention
Showing results 1 to 50 of 50

Collection:

Collection ID:CO003208
Collection Summary:Feces were collected at and stored at -80°C for bile acids analysis.
Sample Type:Feces
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003224
Treatment Summary:Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).

Sample Preparation:

Sampleprep ID:SP003221
Sampleprep Summary:Sample was extracted in 400 μL of methanol, vortexing and shaking for 60 s, add 100 mg of glass beads, and put into a high-throughput tissue grinder, grinding at 55 Hz for 60 s. Repeat the above operation at least twice; ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL of 30% methanol solution, and add it to the assay vial.
Processing Storage Conditions:4℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN005071
Analysis type MS
Chromatography type LC
Chromatography system Shimmadzu LC-30
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Single quadrupole
MS instrument name ABI Sciex 6500+
Ion Mode NEGATIVE
Units μg/g

Chromatography:

Chromatography ID:CH003830
Instrument Name:Shimmadzu LC-30
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:0~9 min, 30% B; 9~14 min, 30~36% B;14~18 min, 36~38% B;18~24 min, 38~50% B;24~32 min, 50~75% B;32~33 min, 75~90% B;33~35.5 min, 90~30% B
Flow Rate:5 μL/min
Solvent A:0.01% formic acid in water
Solvent B:100% acetonitrile
Chromatography Type:LC

MS:

MS ID:MS004809
Analysis ID:AN005071
Instrument Name:ABI Sciex 6500+
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:Electrospray ionization (ESI) source, negative ion ionization mode. Ion source temperature 500°C, ion source voltage -4500 V, collision gas 6 psi, air curtain gas 30 psi, nebulizer gas and auxiliary gas both 50 psi.Scanning was performed using multiple reaction monitoring (MRM). The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.
Ion Mode:NEGATIVE
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