Perform Volcano plot analysis on selected experimental conditions followed by MS search on significant features

Group1Experimental factorGroup2
Sample source:Erastin-resis SNU-668 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-668 cells | transfected:shRNA-SOX23 (1)
Sample source:Erastin-resis SNU-669 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-669 cells | transfected:shRNA-SOX24 (1)
Sample source:Erastin-resis SNU-670 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-671 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-672 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-673 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-674 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-675 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-676 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-677 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-678 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-679 cells | transfected:shRNA-NC (1)
Sample source:Erastin-resis SNU-680 cells | transfected:shRNA-SOX13 (1)
Sample source:Erastin-resis SNU-681 cells | transfected:shRNA-SOX14 (1)
Sample source:Erastin-resis SNU-682 cells | transfected:shRNA-SOX15 (1)
Sample source:Erastin-resis SNU-683 cells | transfected:shRNA-SOX16 (1)
Sample source:Erastin-resis SNU-684 cells | transfected:shRNA-SOX17 (1)
Sample source:Erastin-resis SNU-685 cells | transfected:shRNA-SOX18 (1)
Sample source:Erastin-resis SNU-686 cells | transfected:shRNA-SOX19 (1)
Sample source:Erastin-resis SNU-687 cells | transfected:shRNA-SOX20 (1)
Sample source:Erastin-resis SNU-688 cells | transfected:shRNA-SOX21 (1)
Sample source:Erastin-resis SNU-689 cells | transfected:shRNA-SOX22 (1)

Filtering Parameters
Select threshold % for selection below:
Option 1: No filtering
Option 2: Filter by Sum (Analytes whose sum for all samples falls below the threshold % will be excluded)
Option 3: Filter by Mean (Analytes whose mean for all samples falls below the threshold % will be excluded)
Option 4: Filter by Median (Analytes whose median for all samples falls below the threshold % will be excluded)
Option 5: Filter by Sample threshold       must exceed the above threshold per analyte
In order for an analyte to be retained, the % of samples in one/both groups which are non-zero must exceed the threshold.
Option 6: Remove all analytes containing null values
Sample Normalization Parameters
Normalize samples by:
Volcano Plot Parameters
P-value cutoff: Fold-change cutoff:
MS Search Parameters
Search Refmet database
MS Ion polarity:POSITIVE MS search m/z tolerance:
Ion adducts(choose at least one)
Positive mode:
 [M+H]+ [M+H-H2O]+ [M+Na]+ [M+NH4]+ [M+K]+ [M+2H]2+ [M+2Na]2+ [M+2Na-H]+
[M+H-EtnP]+ [M+H-SerP]+
  logo