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MB Sample ID: SA333420
| Local Sample ID: | 185S0 |
| Subject ID: | SU003215 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003215 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 185S0 | SA333420 | FL039597 | fecal | Sample source |
| 185S0 | SA333420 | FL039597 | control | Factor |
Collection:
| Collection ID: | CO003208 |
| Collection Summary: | Feces were collected at and stored at -80°C for bile acids analysis. |
| Sample Type: | Feces |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR003224 |
| Treatment Summary: | Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks). |
Sample Preparation:
| Sampleprep ID: | SP003221 |
| Sampleprep Summary: | Sample was extracted in 400 μL of methanol, vortexing and shaking for 60 s, add 100 mg of glass beads, and put into a high-throughput tissue grinder, grinding at 55 Hz for 60 s. Repeat the above operation at least twice; ultrasonic at room temperature for 30 min, centrifuged at 12,000 rpm at 4 ℃ for 10 min, and 200 μL of supernatant mixed with 400 μL of water. Take 200 μL of supernatant and add 400 μL of water, vortex for 30 s; take 300 μL of supernatant and filter it through 0.22 μm membrane, vortex for 30 s. After centrifugation, take 20 μL of supernatant and dilute it 10 times with 180 μL of 30% methanol solution, and add it to the assay vial. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN005071 |
|---|---|
| Analysis type | MS |
| Chromatography type | LC |
| Chromatography system | Shimmadzu LC-30 |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | ABI Sciex 6500+ |
| Ion Mode | NEGATIVE |
| Units | μg/g |
Chromatography:
| Chromatography ID: | CH003830 |
| Instrument Name: | Shimmadzu LC-30 |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0~9 min, 30% B; 9~14 min, 30~36% B;14~18 min, 36~38% B;18~24 min, 38~50% B;24~32 min, 50~75% B;32~33 min, 75~90% B;33~35.5 min, 90~30% B |
| Flow Rate: | 5 μL/min |
| Solvent A: | 0.01% formic acid in water |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | LC |
MS:
| MS ID: | MS004809 |
| Analysis ID: | AN005071 |
| Instrument Name: | ABI Sciex 6500+ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| MS Comments: | Electrospray ionization (ESI) source, negative ion ionization mode. Ion source temperature 500°C, ion source voltage -4500 V, collision gas 6 psi, air curtain gas 30 psi, nebulizer gas and auxiliary gas both 50 psi.Scanning was performed using multiple reaction monitoring (MRM). The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment. |
| Ion Mode: | NEGATIVE |
