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MB Sample ID: SA013163
| Local Sample ID: | Stewart_WT_tube_1019_1 |
| Subject ID: | SU000309 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000309 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Stewart_WT_tube_1019_1 | SA013163 | FL003109 | control | Tissue |
Collection:
| Collection ID: | CO000303 |
| Collection Summary: | n/a |
| Sample Type: | maize starchy endosperm |
| Collection Method: | manual removal from maize ear |
| Collection Location: | Iowa State University |
| Storage Conditions: | ear frozen in liquid N2, kernels removed while frozen, and maintained at -80C |
Treatment:
| Treatment ID: | TR000323 |
| Treatment Summary: | Each ear is segregating both mutant and wild type kernels, thus the harvest and freezing for the controls is the exact same as the mutant sibling kernels from the same ear. |
| Plant Growth Location: | Woodruff Farm, Story Co., Ames, IA |
| Plant Plot Design: | 10 plants/row; random row placement over 0.5 acres |
| Plant Light Period: | natural sunlight and day length, summer 2014 |
| Plant Estab Date: | 2014-05-01 |
| Plant Growth Stage: | 20 days after pollination |
| Plant Metab Quench Method: | flash frozen in liquid N2, maintained at -80C |
Sample Preparation:
| Sampleprep ID: | SP000317 |
| Sampleprep Summary: | n/a |
| Sampleprep Protocol Filename: | Plant_Tissue.pdf |
Combined analysis:
| Analysis ID | AN000460 | AN000461 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000328 |
| Methods Filename: | Instrument_Parameters.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000400 |
| Analysis ID: | AN000460 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
| MS ID: | MS000401 |
| Analysis ID: | AN000461 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
