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MB Sample ID: SA025640
Local Sample ID: | 141217actsa01_1 |
Subject ID: | SU000516 |
Subject Type: | Animal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Genotype Strain: | Hound-type |
Age Or Age Range: | Adult |
Weight Or Weight Range: | 35-60 lbs |
Gender: | Male/Female |
Animal Inclusion Criteria: | no evidence of gastrointestinal illness or other disease |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000523 |
Sampleprep Summary: | 1. Weigh 50 mg tissue sample in to a 25 ml conical polypropylene centrifuge tube. 2. Add 2.5mL extraction solvent to the tissue sample and homogenize for 45 seconds ensuring that sample resembles a powder. In between samples, clean the homogenizer in solutions of methanol, acetone, water, and the extraction solvent. 3. Centrifuge the samples at 2500 rpm. for 5 minutes. Aliquot 2 X 500μl supernatant, one for analysis and one for a backup sample. Store backup aliquot in the -20°C freezer. 4. Evaporate one 500μl aliquot of the sample in the Labconco Centrivap cold trap concentrator to complete dryness 5. The dried aliquot is then re-suspended with 500l 50% acetonitrile (degassed as given) 6. Centrifuge for 2 min at 14000 rcf using the centrifuge Eppendorf 5415. 7. Remove supernatant to a new Eppendorff tube. 8. Evaporate the supernatant to dryness in the the Labconco Centrivap cold trap concentrator. 9. Submit to derivatization. |