Summary of Study ST003091
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001920. The data can be accessed directly via it's Project DOI: 10.21228/M8JB09 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003091 |
Study Title | T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia |
Study Type | Untargeted metabolomics |
Study Summary | We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models. |
Institute | Translational Genomics Research Institute |
Department | Integrated Mass Spectrometry Shared Resources |
Last Name | Pirrotte |
First Name | Patrick |
Address | 445 N 5th St Phoenix, AZ 85004 |
ppirrotte@tgen.org | |
Phone | 6023438400 |
Submit Date | 2024-01-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-03-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005056 | AN005057 | AN005058 | AN005059 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer | Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak area | Peak area | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH003819 |
Chromatography Summary: | Reversed phase chromatography |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) |
Column Temperature: | 45 |
Flow Gradient: | 0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min |
Flow Rate: | 0.3ml/min |
Injection Temperature: | 4 |
Solvent A: | Water, 0.1% formic acid |
Solvent B: | Acetonitrile, 0.1% formic acid |
Transferline Temperature: | 275 |
Target Sample Temperature: | 4 |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003820 |
Chromatography Summary: | HILIC chromatography |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min |
Flow Rate: | 0.35ml/min |
Injection Temperature: | 4 |
Solvent A: | 10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid |
Solvent B: | 10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid |
Transferline Temperature: | 275 |
Target Sample Temperature: | 4 |
Chromatography Type: | HILIC |