Summary of Study ST003114
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001935. The data can be accessed directly via it's Project DOI: 10.21228/M8M430 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003114 |
| Study Title | Lipidomics analyses in model membranes, isolated mitochondria and cellular systems to study how the local lipid environment affects BAX and BAK function during apoptosis. |
| Study Summary | To investigate how the local lipid environment affects BAX and BAK function during apoptosis, we performed quantitative analyses of different lipid classes (glycerophospholipids, fatty acids, ceramides and sphingomyelins) in cultured cells, isolated mitochondria and lipid nanodics formed by Styrene-Malic Acid (SMA) co-polymers. Ceramides, sphingomyelins, fatty acids and cardiolipins were analyzed by Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS). For glycerophospholipids (PC, PE, PI, PS, PG, PA) we applied direct infusion MS approaches (Shotgun Lipidomics). |
| Institute | University of Cologne |
| Department | Faculty of Medicine and University Hospital of Cologne, Cluster of Excellence Cellular Stress Responses in Aging-associated Diseases (CECAD) |
| Laboratory | CECAD Lipidomics/Metabolomics Facility |
| Last Name | Brodesser |
| First Name | Susanne |
| Address | Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany |
| susanne.brodesser@uk-koeln.de | |
| Phone | +49 221 478 84015 |
| Submit Date | 2023-08-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005102 | AN005103 | AN005104 | AN005105 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | None (Direct infusion) | Normal phase | Reversed phase | Reversed phase |
| Chromatography system | Advion TriVersa NanoMate | Agilent 1260 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | None | Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) | Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) | Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTRAP | QTRAP | QTRAP | QTRAP |
| MS instrument name | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE |
| Units | counts per second (cps) | counts per second (cps) | counts per second (cps) | counts per second (cps) |
Chromatography:
| Chromatography ID: | CH003858 |
| Chromatography Summary: | Lipid extract infusion and ionization was conducted using Nano-ESI chips with the TriVersa NanoMate operated by the ChipSoft Software (Advion) under the following settings: sample infusion volume: 14 μl, volume of air to aspirate after sample: 1 μl, air gap before chip: enabled, aspiration delay: 0 s, prepiercing: with mandrel, spray sensing: enabled, cooling temperature: 14°C, gas pressure: 0.5 psi, ionization voltage: 1.4 kV, and vent headspace: enabled. Prewetting was done once. |
| Instrument Name: | Advion TriVersa NanoMate |
| Column Name: | None |
| Column Temperature: | N/A |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | None |
| Solvent B: | None |
| Chromatography Type: | None (Direct infusion) |
| Chromatography ID: | CH003859 |
| Chromatography Summary: | Note: Macherey-Nagel does not offer this column as standard, but manufactures it on request. |
| Instrument Name: | Agilent 1260 |
| Column Name: | Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) |
| Column Temperature: | 20 |
| Flow Gradient: | 0 min: 0% B, 0.5 min: 0% B, 0.7 min: 10% B, 1.2 min: 10% B, 1.6 min: 18% B, 2.2 min: 18% B, 2.6 min: 100% B, 4.5 min: 100% B, 4.9 min: 0% B, 6.5 min: 0% B |
| Flow Rate: | 0.75 ml/min |
| Solvent A: | 97% acetonitrile/2% methanol/1% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 99% methanol/1% acetic acid; 5 mM ammonium acetate |
| Chromatography Type: | Normal phase |
| Chromatography ID: | CH003860 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min: 30% B, 0.5 min: 30% B, 4.5 min: 68% B, 20.5 min: 75% B, 21 min: 97% B, 24 min: 97% B, 24.5 min: 30% B, 28 min: 30% B |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003861 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min: 55% B, 4 min: 95% B, 7 min: 95% B, 7.1 min: 55% B, 10 min: 55% B |
| Flow Rate: | 0.7 ml/min |
| Solvent A: | 100% water; 0.012 % acetic acid; 5 mM ammonium acetate |
| Solvent B: | 80% acetonitrile/20% isopropanol |
| Chromatography Type: | Reversed phase |
