Summary of Study ST000887
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000616. The data can be accessed directly via it's Project DOI: 10.21228/M89X34 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000887 |
| Study Title | Evaluation of Quenching and Extraction Procedures for Performing Metabolomics in Acidithiobacillus ferrooxidans |
| Study Summary | To perform a Metabolomic Analysis of At. ferrooxidans during its early exponential growth phase as well as its late exponential growth phase. |
| Institute | Laurentian University |
| Last Name | Doran |
| First Name | Marney |
| Address | Laurentian University |
| mdoran@laurentian.ca | |
| Phone | 8196740310 |
| Submit Date | 2017-10-26 |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-11-10 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001163 |
| Collection Summary: | Replicate cultures of Acidithiobacillus ferrooxidans (American Type Culture Collection strain 23270) were grown in 1 L of Tuovinen and Kelly (1973) liquid medium ([FeSO4·7H2O] = 33.4 g/L; 0.3 % (w/v) (NH4)2SO4; MgSO4·7H2O; K2HPO4, pH 2.5). Cells were incubated on a gyratory shaker at 180 rpm and 30 °C. Cells harvested for the metabolomic comparison of two points in the growth curve were in late exponential growth phase (OD425 = 0.85) or in early exponential growth phase (OD425 = 0.65). The cells were harvested via centrifugation at 14,000 x g for 5 min at 4 °C then transferred into a 1.5 mL centrifuge tube. |
| Sample Type: | Bacterial cells |
