Summary of Study ST003152
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003152 |
Study Title | Metabolomics analysis of murine xenograft tumors derived from human breast cancer cell line MCF7 1 h after isotopic glucose bolus |
Study Type | Intracellular metabolomics, [U-13C6] glucose tracing |
Study Summary | Metabolomics analysis of murine xenograft tumors derived from human breast cancer cell line MCF7 1 h after isotopic glucose bolus. The NSG female mice were ~55 days after grafting tumors. [U-13C6] D-Glucose bolus was delivered intraperitoneally with a 10 mg/kg dose of Fasnall. |
Institute | Wistar Institute |
Department | Molecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center |
Laboratory | Schug's Lab |
Last Name | Mukha |
First Name | Dzmitry |
Address | 3601 Spruce St, Philadelphia, PA 19104, USA |
dmukha@wistar.org | |
Phone | 2154956903 |
Submit Date | 2024-03-09 |
Num Groups | 2 |
Total Subjects | 12 |
Num Females | 12 |
Publications | Submission Pending |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML, raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-04-02 |
Release Version | 1 |
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Collection:
Collection ID: | CO003262 |
Collection Summary: | Tissue metabolite extracts were prepared from ~50 mg snap-frozen tissue samples. Each sample was weighed while frozen. Frozen samples were ground at the temperature of liquid nitrogen by Retch Cryomill. Then, 80% methanol was added to each tube at a ratio of 1 ml per 50 mg of tissue. |
Collection Protocol Filename: | DM_metabolomics_samples.txt |
Sample Type: | Tumor cells |
Collection Method: | 80% methanol extraction |
Volumeoramount Collected: | ~1 ml |
Storage Conditions: | -80℃ |
Collection Vials: | 1.5 ml plastic centrifuge tubes |
Storage Vials: | 1.5 ml plastic centrifuge tubes |