Summary of Study ST002994

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001864. The data can be accessed directly via it's Project DOI: 10.21228/M8S425 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study ID	ST002994
Study Title	Integrating uterine microbiome and metabolome to advance the understanding of the uterine environment in dairy cows with metritis
Study Summary	Background: Metritis is a prevalent uterine disease that affects the welfare, fertility, and survival of dairy cows. The uterine microbiome from cows that develop metritis and those that remain healthy do not differ from calving until 2 days after calving, after which there is a dysbiosis of the uterine microbiome characterized by a shift towards opportunistic pathogens such as Fusobacteriota and Bacteroidota. Whether these opportunistic pathogens proliferate and overtake the uterine commensals could be determined by the type of substrates present in the uterus. The objective of this study was to integrate uterine metabolome and microbiome data to advance the understanding of metritis development in dairy cows. Holstein cows (n = 104) had uterine fluid collected at calving and at the day of metritis diagnosis. Cows with metritis (n = 52) were paired with cows without metritis (n = 52) based on days after calving. First, the uterine metabolome and microbiome were evaluated individually, and then integrated using network analyses. Results: The uterine metabolome differed both at calving and on the day of metritis diagnosis between cows with and without metritis. The uterine microbiome did not differ at calving but differed on the day of metritis diagnosis between cows with and without metritis. Omics integration was performed between 153 significant metabolites and 6 significant bacteria genera on the day of metritis diagnosis. A total of 49 metabolites were correlated with 3 bacteria genera (i.e. Fusobacteria, Porphyromonas and Bacteroides) on the day of metritis diagnosis. The main metabolites have been associated with attenuation of biofilm formation by commensal bacteria, pathogenic bacterial overgrowth, defense mechanisms against the immune system, tissue damage and inflammation, and immune dysregulation. Conclusions: The data integration presented herein helps advance the understanding of metritis development in dairy cows. The identified metabolites may be promising targets for future interventions aiming to reduce pathogenic bacterial growth in the uterus, and therefore, reducing the incidence of metritis.
Institute	University of Florida
Last Name	Casaro
First Name	Segundo
Address	117 Deriso Hall, 2015 SW 16th Ave., Gainesville, FL 32610
Email	segundocasaro@ufl.edu
Phone	3522844016
Submit Date	2023-11-30
Analysis Type Detail	GC-MS
Release Date	2024-03-01
Release Version	1

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Factors:

Subject type: Mammal; Subject species: Bos taurus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Time	Parity
SA325945	9971_Calving_Con	Con	Calving	Mult
SA325946	9918_Calving_Con	Con	Calving	Mult
SA325947	9917_Calving_Con	Con	Calving	Mult
SA325948	9907_Calving_Con	Con	Calving	Mult
SA325949	9979_Calving_Con	Con	Calving	Mult
SA325950	9924_Calving_Con	Con	Calving	Mult
SA325951	9966_Calving_Con	Con	Calving	Mult
SA325952	9956_Calving_Con	Con	Calving	Mult
SA325953	9927_Calving_Con	Con	Calving	Mult
SA325954	9957_Calving_Con	Con	Calving	Mult
SA325955	9925_Calving_Con	Con	Calving	Mult
SA325956	9944_Calving_Con	Con	Calving	Mult
SA325957	9904_Calving_Con	Con	Calving	Mult
SA325958	9872_Calving_Con	Con	Calving	Mult
SA325959	9823_Calving_Con	Con	Calving	Mult
SA325960	9804_Calving_Con	Con	Calving	Mult
SA325961	10038_Calving_Con	Con	Calving	Mult
SA325962	10001_Calving_Con	Con	Calving	Mult
SA325963	9992_Calving_Con	Con	Calving	Mult
SA325964	9876_Calving_Con	Con	Calving	Mult
SA325965	9985_Calving_Con	Con	Calving	Mult
SA325966	9987_Calving_Con	Con	Calving	Mult
SA325967	9875_Calving_Con	Con	Calving	Mult
SA325968	9509_Calving_Con	Con	Calving	Mult
SA325969	9942_Calving_Con	Con	Calving	Mult
SA325970	9268_Calving_Con	Con	Calving	Mult
SA325971	9536_Calving_Con	Con	Calving	Mult
SA325972	9778_Calving_Con	Con	Calving	Mult
SA325973	9794_Calving_Con	Con	Calving	Mult
SA325974	9280_Calving_Con	Con	Calving	Mult
SA325975	9802_Calving_Con	Con	Calving	Mult
SA325976	9954_Calving_Con	Con	Calving	Mult
SA325977	9945_Calving_Con	Con	Calving	Mult
SA325978	9950_Calving_Con	Con	Calving	Mult
SA325979	10311_Calving_Con	Con	Calving	Prim
SA325980	10165_Calving_Con	Con	Calving	Prim
SA325981	10290_Calving_Con	Con	Calving	Prim
SA325982	10171_Calving_Con	Con	Calving	Prim
SA325983	10229_Calving_Con	Con	Calving	Prim
SA325984	10303_Calving_Con	Con	Calving	Prim
SA325985	10274_Calving_Con	Con	Calving	Prim
SA325986	10199_Calving_Con	Con	Calving	Prim
SA325987	10246_Calving_Con	Con	Calving	Prim
SA325988	10245_Calving_Con	Con	Calving	Prim
SA325989	10212_Calving_Con	Con	Calving	Prim
SA325990	10268_Calving_Con	Con	Calving	Prim
SA325991	10194_Calving_Con	Con	Calving	Prim
SA325992	10264_Calving_Con	Con	Calving	Prim
SA325993	10262_Calving_Con	Con	Calving	Prim
SA325994	10189_Calving_Con	Con	Calving	Prim
SA325995	10261_Calving_Con	Con	Calving	Prim
SA325996	10254_Calving_Con	Con	Calving	Prim
SA325997	9924_Diagnosis_Con	Con	Diagnosis	Mult
SA325998	9804_Diagnosis_Con	Con	Diagnosis	Mult
SA325999	9536_Diagnosis_Con	Con	Diagnosis	Mult
SA326000	9802_Diagnosis_Con	Con	Diagnosis	Mult
SA326001	9945_Diagnosis_Con	Con	Diagnosis	Mult
SA326002	9778_Diagnosis_Con	Con	Diagnosis	Mult
SA326003	9509_Diagnosis_Con	Con	Diagnosis	Mult
SA326004	9794_Diagnosis_Con	Con	Diagnosis	Mult
SA326005	9872_Diagnosis_Con	Con	Diagnosis	Mult
SA326006	9907_Diagnosis_Con	Con	Diagnosis	Mult
SA326007	9904_Diagnosis_Con	Con	Diagnosis	Mult
SA326008	9268_Diagnosis_Con	Con	Diagnosis	Mult
SA326009	9985_Diagnosis_Con	Con	Diagnosis	Mult
SA326010	9823_Diagnosis_Con	Con	Diagnosis	Mult
SA326011	9280_Diagnosis_Con	Con	Diagnosis	Mult
SA326012	9875_Diagnosis_Con	Con	Diagnosis	Mult
SA326013	9876_Diagnosis_Con	Con	Diagnosis	Mult
SA326014	9954_Diagnosis_Con	Con	Diagnosis	Mult
SA326015	9917_Diagnosis_Con	Con	Diagnosis	Mult
SA326016	9918_Diagnosis_Con	Con	Diagnosis	Mult
SA326017	9971_Diagnosis_Con	Con	Diagnosis	Mult
SA326018	9966_Diagnosis_Con	Con	Diagnosis	Mult
SA326019	9979_Diagnosis_Con	Con	Diagnosis	Mult
SA326020	9987_Diagnosis_Con	Con	Diagnosis	Mult
SA326021	10001_Diagnosis_Con	Con	Diagnosis	Mult
SA326022	9992_Diagnosis_Con	Con	Diagnosis	Mult
SA326023	9957_Diagnosis_Con	Con	Diagnosis	Mult
SA326024	9956_Diagnosis_Con	Con	Diagnosis	Mult
SA326025	9927_Diagnosis_Con	Con	Diagnosis	Mult
SA326026	9925_Diagnosis_Con	Con	Diagnosis	Mult
SA326027	9942_Diagnosis_Con	Con	Diagnosis	Mult
SA326028	9944_Diagnosis_Con	Con	Diagnosis	Mult
SA326029	9950_Diagnosis_Con	Con	Diagnosis	Mult
SA326030	10038_Diagnosis_Con	Con	Diagnosis	Mult
SA326031	10165_Diagnosis_Con	Con	Diagnosis	Prim
SA326032	10212_Diagnosis_Con	Con	Diagnosis	Prim
SA326033	10229_Diagnosis_Con	Con	Diagnosis	Prim
SA326034	10245_Diagnosis_Con	Con	Diagnosis	Prim
SA326035	10311_Diagnosis_Con	Con	Diagnosis	Prim
SA326036	10246_Diagnosis_Con	Con	Diagnosis	Prim
SA326037	10189_Diagnosis_Con	Con	Diagnosis	Prim
SA326038	10199_Diagnosis_Con	Con	Diagnosis	Prim
SA326039	10268_Diagnosis_Con	Con	Diagnosis	Prim
SA326040	10290_Diagnosis_Con	Con	Diagnosis	Prim
SA326041	10254_Diagnosis_Con	Con	Diagnosis	Prim
SA326042	10264_Diagnosis_Con	Con	Diagnosis	Prim
SA326043	10274_Diagnosis_Con	Con	Diagnosis	Prim
SA326044	10303_Diagnosis_Con	Con	Diagnosis	Prim