Summary of Study ST000212
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000178. The data can be accessed directly via it's Project DOI: 10.21228/M8VS3Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000212 |
Study Title | Murine gut bile acid analysis |
Study Type | Changes to bile acids in the murine gut after antibiotics Bile acid analysis (colon contents/plasma) |
Study Summary | Samples were collected at the time of necropsy. Contents from the lumen of the gastrointestinal tract of C57BL/6 mice were collected, flash frozen and stored in the -80C until processing for metabolomics assay. C57BL/6 mice treated with cefoperazone for 5 days with two days of water (n=5) were harvested along with non-antibiotic treated mice (n=5). Contents from the small intesine (duodenum, jejunum and ileum), the large intestine (cecum, colon) and stool were collected for targeted bile acid analysis. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333439/ |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-12 |
Num Groups | 12 |
Total Subjects | 60 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 32 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-12-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000314 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | NEGATIVE |
Units | µg/100mg sample |
MS:
MS ID: | MS000263 |
Analysis ID: | AN000314 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Acquisition Parameters File: | 2014-03-27-Bile_acid-full-ESN-insert.m.zip |
Processing Parameters File: | EX00256-Bile-Acids-Quant-method.m.zip |