Summary of Study ST001713
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001097. The data can be accessed directly via it's Project DOI: 10.21228/M8WT3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001713 |
Study Title | Effects of different planting densities on the metabolism of Panax notoginseng |
Study Type | Planting density experiment |
Study Summary | At the moderate planting density, the primary metabolism (starch and sucrose metabolism) of the plants were significantly enhanced. However, the strong intraspecific competition at the higher planting densities resulted in stress as well as the accumulation of antioxidants (gentiobiose, oxalic acid, dehydroascorbic acid) and other stress resistance-related metabolites. Interestingly, the planting at low densities with low intraspecific competition disturbed normal carbohydrate metabolism by upregulating galactose metabolism. |
Institute | Yunnan Agricultural University |
Department | College of Plant Protection |
Laboratory | Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education |
Last Name | Haijiao |
First Name | Liu |
Address | 452 Fengyuan road, Kunming, Yunnan, China |
15832256149@163.com | |
Phone | +8615288149641 |
Submit Date | 2021-01-25 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2022-01-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001796 |
Sampleprep Summary: | Approximately 60 mg of frozen powder fibrous roots and 1 mL of methanol (CH3OH) containing 0.5 mg of ribitol were added into a prechilled 2 mL lock-cap centrifuge tube, then vortexed for 10 s. A 300 μL extraction aliquot (H2O:methanol:chloroform=1:2.5:1, v:v:v) was added and ultrasonically extracted for 30 min at 37°C. Then, the sample was centrifuged (1600 g, 3 min) to separate the polar and nonpolar phases. Then the upper polar phase was transferred to a fresh centrifuge tube and added 200 μL sterile water, and then vortexed and centrifuged (1600 g, 4°C for 3 min). A 250 μL aliquot of the upper phase was transferred to a fresh centrifuge tube, dried for 3-4 h at room temperature using a SpeedVac (Christ, Germany). Adding 80 μL of methoxyamine hydrochloride solution (20 mg mL-1 dissolved in pyridine) to each sample and incubating for 90 min at 30°C to protect carbonyl moieties. And then, 40 μL N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) was added and incubating at 37°C for 30 min to trimethylsilylate the acidic protons. After this step, the sample was centrifuged (1600 g, 4°C for 3 min), then the supernatant was stored at 4°C for further analysis. |