Summary of Study ST002502
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001616. The data can be accessed directly via it's Project DOI: 10.21228/M8TB15 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002502 |
| Study Title | The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (IKIDS Cohort) |
| Study Type | Prospective Cohort Study |
| Study Summary | IKIDS is a prospective birth cohort in Central Illinois. Pregnant women were recruited at two obstetrical clinics in Urbana Champaign IL from 2014-2021. Women were enrolled between 8-14 weeks of pregnancy. Eligibility criteria included 18-40 years of age, English speaking, not in a high risk pregnancy, planning to stay in the area until their child's first birth day and living within 30 minutes of the University of Illinois campus. Health, demographic and life style data and biospecimens were collected at 5 time points across pregnancy and children were followed prospectively from birth including assessments at birth, 1-5 weeks 4.5 months, 7.5 months, 2 years, 3 years, 4 years and 7.5 years of age. Physical and neurobehavioral development were assessed, with a particular focus on the impacts of prenatal chemical exposures (phthalates, phenols, PFAS) or prenatal maternal stress on child health outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. IKIDS is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539 |
| Institute | University of Illinois Urbana-Champaign |
| Department | Beckman Institute for Advanced Science and Technology |
| Last Name | Schantz |
| First Name | Susan |
| Address | 2325/21 Beckman Institute 405 North Mathews Avenue Urbana, IL 61801 |
| schantz@illinois.edu | |
| Phone | (217) 333-6230 |
| Submit Date | 2023-03-08 |
| Total Subjects | 300 |
| Study Comments | HHEAR Project EM20-0011, ECHO Project EC0376 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002605 |
| Sampleprep Summary: | Study samples and study pools (50 µL aliquots) were shipped from the University of Illinois at Urbana-Champaign to the NC HHEAR Hub on dry ice. HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used as blanks. All samples were thawed at 4°C overnight before the preparation. Samples, including study samples, study pool samples, HHEAR reference urine, CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS. |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Water-Methanol (95:5, v/v) |
| Sample Spiking: | Tryptophan-d5 stock solution at 500 ng/mL |
