Summary of Study ST002692
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001667. The data can be accessed directly via it's Project DOI: 10.21228/M87727 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002692 |
| Study Title | Per- and poly-fluoroalkyl substances (PFAS) Exposures and Child Health (PEACH) Study: Using targeted exposure assessment and untargeted metabolic profiling to characterize molecular pathways and mechanisms underlying PFAS toxicity on adverse birth and child health outcomes |
| Study Type | Prospective Cohort Study |
| Study Summary | The overarching goal of the Per- and poly-fluoroalkyl substances Exposures And Child Health (PEACH) Study is to apply an advanced untargeted metabolomics workflow to investigate associations between PFAS levels, perturbations in maternal and newborn metabolome and adverse birth outcomes. The Emory ECHO team has established a socio-economically diverse, exceptionally phenotyped African American (AA) maternal-child cohort that enrolls pregnant women in the early prenatal period and extends dyad follow-up through age five. PEACH draws from repeated metabolic profiling on a subset of 320 AA pregnant people within the Atlanta ECHO cohort, PFAS assessment, and untargeted metabolomics from newborn blood spots (n=279). Please contact Drs. Donghai Liang (Donghai.liang@emory.edu) and Anne Dunlop (amlang@emory.edu) via email for questions related to the subject characteristics and outcomes. This research was supported by the Environmental influences on Child Health Outcomes (ECHO) OIF program, Office of The Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. PEACH is an ECHO cohort which is supported by the following ECHO Program Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data Analysis Center: Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier D; U24 OD023382 North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ES026539 |
| Institute | Emory University |
| Department | Environmental Health |
| Last Name | Liang |
| First Name | Donghai |
| Address | 1518 Clifton Rd. NE Atlanta, GA 30322 |
| donghai.liang@emory.edu | |
| Phone | (404) 712-9583 |
| Submit Date | 2023-04-26 |
| Total Subjects | 279 |
| Study Comments | HHEAR Project EO19-0009, ECHO Project EC0386 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002800 |
| Sampleprep Summary: | 279 newborn dried blood spot and blank samples and were randomized and extracted by 1000 µL methanol with 500 ng/mLtryptophan-d5 as internal standard via a multiple tube vortex mixer for 10 minutes at 5000 rpm. The blank and study samples were sonicated in an ice bath for 20 minutes. A volume of 700 µL of the supernatants was transferred into a pre-labeled 2.0 mL Low-bind Eppendorf tube labeled as the stock. Protein precipitate was pelleted using a centrifuge operating at 4° C for 5 minutes at 16,000 rcf. A volume of 70 µL of each blank sample stock was transferred to 15 mL conical tube to be combined to create a Blank Pool (BP). A volume of 70 µL of each study sample stock was transferred to 15 mL conical tube to be combined and create a Study Pool (SP). A total volume of 1400 µL of NIST Reference Plasma (NIST) was aliquoted into twenty-eight 50 µL aliquots. A volume of 600 µL supernatant (including experimental samples, BP, and SP) was transferred into a pre-labeled 2.0 mL Low-bind Eppendorf tube. All samples and pools were then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was added to reconstitute the dried extracts, and the samples were thoroughly mixed on multiple tube vortex mixer for 10 min at 5000 rpm. Samples were centrifuged at 4°C for 4 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS. Broad-spectrum metabolomics was conducted using a Thermo Scientific™ Vanquish™ UPHPLC - Q Exactive™ HF-X Orbitrap System. Metabolites were separated on an Acquity UPLC HSS T3 C18 (2.1 X 100 mm, 1.8 µm) operating at 50 C using a reversed phase gradient separation with Water with 0.1% Formic Acid (v/v) as mobile phase A and Methanol with 0.1% Formic Acid (v/v) as mobile phase B. A 5 µL was injected into the instrument, and MS was collected between 50-750 m/z in positive mode with the MS/MS data triggered by the Data-Dependent Acquisition. |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Water-Methanol (95:5, v/v) |
| Sample Spiking: | Tryptophan-d5 stock solution at 500 ng/mL |
