Summary of Study ST003129

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001943. The data can be accessed directly via it's Project DOI: 10.21228/M8K43P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003129
Study TitlePulmonary maternal immune activation does not extend through the placenta but leads to fetal metabolic adaptation - Maternal liver
Study SummaryMaternal immune system activation (MIA) during pregnancy can disrupt the fetal environment, causing postnatal susceptibility to disorders. How the placenta and the fetus respond to acute MIA over time is unknown. Here, we characterized the response to acute maternal pulmonary inflammation across time in maternal and fetal organs using multi-omics. Unlike maternal organs which mounted strong innate immune responses, the placenta upregulated tissue-integrity genes, likely to prevent fetal exposure to infections, and downregulated growth-associated genes. Subsequently, the placenta upregulated biosynthesis and endoplasmic reticulum stress genes in order to return to homeostasis. These responses likely protected the fetus, since we observed no immune response in fetal liver. Instead, likely due to nutrient depletion, the fetal liver displayed metabolic adaptations, including increases in lipids containing docosahexaenoic acid, crucial for fetal brain development. Our study shows, for the first time, the integrated temporal response to pulmonary MIA across maternal and fetal organs.
Institute
University of Copenhagen
DepartmentDepartment of Biology
LaboratorySection for Computational and RNA Biology
Last NameSandelin
First NameAlbin
AddressCopenhagen University Ole Maaloes Vej 5, DK2200, Copenhagen N, Denmark
Emailalbin@binf.ku.dk
Phone+45 224 56668
Submit Date2024-03-11
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-04-02
Release Version1
Albin Sandelin Albin Sandelin
https://dx.doi.org/10.21228/M8K43P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP003253
Sampleprep Summary:Lipids were extracted from fetal liver samples (20 mg) using Folch extraction* with 8-12 replicates from each experimental group at each time point. Prior to tissue lysis, Splash mix (Merck) was added to the extraction solvent and tissue samples were lysed by beat beating in a FastPrep-24 homogenizer. After centrifugation and phase separation, the apolar and polar phases were transferred to separate tubes, and the apolar phase dried under N2. *Folch, J., Lees, M. & Sloane Stanley, G. H. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509 (1957).
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