Summary of Study ST000212

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000178. The data can be accessed directly via it's Project DOI: 10.21228/M8VS3Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000212
Study TitleMurine gut bile acid analysis
Study TypeChanges to bile acids in the murine gut after antibiotics Bile acid analysis (colon contents/plasma)
Study SummarySamples were collected at the time of necropsy. Contents from the lumen of the gastrointestinal tract of C57BL/6 mice were collected, flash frozen and stored in the -80C until processing for metabolomics assay. C57BL/6 mice treated with cefoperazone for 5 days with two days of water (n=5) were harvested along with non-antibiotic treated mice (n=5). Contents from the small intesine (duodenum, jejunum and ileum), the large intestine (cecum, colon) and stool were collected for targeted bile acid analysis. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333439/
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-12
Num Groups12
Total Subjects60
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size32 M
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VS3Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000178
Project DOI:doi: 10.21228/M8VS3Q
Project Title:Bile Acid Studies in Murine Gut
Project Summary:Targeted bile acid analysis throughout the murine GI tract before and after antibiotics
Institute:University of Michigan
Department:Int Med-Infectious Diseases
Laboratory:Theriot Lab
Last Name:Theriot
First Name:Casey
Address:Ann Arbor, MI
Email:caseythe@umich.edu
Phone:734-763-4133

Subject:

Subject ID:SU000231
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GI tract location
SA010429S00012887cecum | treatment:cefoperazone
SA010430S00012884cecum | treatment:cefoperazone
SA010431S00012885cecum | treatment:cefoperazone
SA010432S00012886cecum | treatment:cefoperazone
SA010433S00012883cecum | treatment:cefoperazone
SA010434S00012914cecum | treatment:vehicle
SA010435S00012917cecum | treatment:vehicle
SA010436S00012916cecum | treatment:vehicle
SA010437S00012915cecum | treatment:vehicle
SA010438S00012913cecum | treatment:vehicle
SA010439S00012891colon | treatment:cefoperazone
SA010440S00012890colon | treatment:cefoperazone
SA010441S00012889colon | treatment:cefoperazone
SA010442S00012888colon | treatment:cefoperazone
SA010443S00012892colon | treatment:cefoperazone
SA010444S00012922colon | treatment:vehicle
SA010445S00012919colon | treatment:vehicle
SA010446S00012920colon | treatment:vehicle
SA010447S00012921colon | treatment:vehicle
SA010448S00012918colon | treatment:vehicle
SA010449S00012868duodenum | treatment:cefoperazone
SA010450S00012872duodenum | treatment:cefoperazone
SA010451S00012869duodenum | treatment:cefoperazone
SA010452S00012870duodenum | treatment:cefoperazone
SA010453S00012871duodenum | treatment:cefoperazone
SA010454S00012901duodenum | treatment:vehicle
SA010455S00012902duodenum | treatment:vehicle
SA010456S00012899duodenum | treatment:vehicle
SA010457S00012898duodenum | treatment:vehicle
SA010458S00012900duodenum | treatment:vehicle
SA010459S00012880ileum | treatment:cefoperazone
SA010460S00012878ileum | treatment:cefoperazone
SA010461S00012881ileum | treatment:cefoperazone
SA010462S00012879ileum | treatment:cefoperazone
SA010463S00012882ileum | treatment:cefoperazone
SA010464S00012909ileum | treatment:vehicle
SA010465S00012910ileum | treatment:vehicle
SA010466S00012911ileum | treatment:vehicle
SA010467S00012908ileum | treatment:vehicle
SA010468S00012912ileum | treatment:vehicle
SA010469S00012874jejunum | treatment:cefoperazone
SA010470S00012876jejunum | treatment:cefoperazone
SA010471S00012873jejunum | treatment:cefoperazone
SA010472S00012875jejunum | treatment:cefoperazone
SA010473S00012877jejunum | treatment:cefoperazone
SA010474S00012907jejunum | treatment:vehicle
SA010475S00012903jejunum | treatment:vehicle
SA010476S00012904jejunum | treatment:vehicle
SA010477S00012905jejunum | treatment:vehicle
SA010478S00012906jejunum | treatment:vehicle
SA010479S00012897stool | treatment:cefoperazone
SA010480S00012896stool | treatment:cefoperazone
SA010481S00012893stool | treatment:cefoperazone
SA010482S00012894stool | treatment:cefoperazone
SA010483S00012895stool | treatment:cefoperazone
SA010484S00012927stool | treatment:vehicle
SA010485S00012926stool | treatment:vehicle
SA010486S00012923stool | treatment:vehicle
SA010487S00012924stool | treatment:vehicle
SA010488S00012925stool | treatment:vehicle
Showing results 1 to 60 of 60

Collection:

Collection ID:CO000219
Collection Summary:-
Sample Type:Cecum

Treatment:

Treatment ID:TR000239

Sample Preparation:

Sampleprep ID:SP000233
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000314
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units µg/100mg sample

Chromatography:

Chromatography ID:CH000235
Methods ID:AQM040
Methods Filename:2014-03-27-Bile_acid-full-ESN-insert.m.zip

MS:

MS ID:MS000263
Analysis ID:AN000314
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:2014-03-27-Bile_acid-full-ESN-insert.m.zip
Processing Parameters File:EX00256-Bile-Acids-Quant-method.m.zip
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