Summary of Study ST003091

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001920. The data can be accessed directly via it's Project DOI: 10.21228/M8JB09 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003091
Study TitleT cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Study TypeUntargeted metabolomics
Study SummaryWe report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute
Translational Genomics Research Institute
DepartmentIntegrated Mass Spectrometry Shared Resources
Last NamePirrotte
First NamePatrick
Address445 N 5th St Phoenix, AZ 85004
Emailppirrotte@tgen.org
Phone6023438400
Submit Date2024-01-26
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-03-01
Release Version1
Patrick Pirrotte Patrick Pirrotte
https://dx.doi.org/10.21228/M8JB09
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001920
Project DOI:doi: 10.21228/M8JB09
Project Title:T cell Immune Escape in Blast Crisis Transformation of Chronic Myeloid Leukemia
Project Type:Untargeted metabolomics
Project Summary:We report that leukemia secreted cytokines reduce miR-142 levels in T cells, causing T cell loss and exhaustion and therefore promoting CML BC transformation. Our homemade synthetic miR-142 increased T cell antileukemic activity and significantly prolonged the survival of CML BC murine and PDX models.
Institute:Translational Genomics Research Institute
Department:Integrated Mass Spectrometry Shared Resources
Last Name:Pirrotte
First Name:Patrick
Address:445 N 5th St Phoenix, AZ 85004
Email:ppirrotte@tgen.org
Phone:6023438400
Contributors:Bin Zhang, Khyatiben V. Pathak, Brooke Lovell, Nate Hansen, Patrick Pirrote, Le Xuan Truong Nguyen, Guido Marcucci,

Subject:

Subject ID:SU003206
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype
SA332985Norm. Area 088_0009_01B_R_TM48xWTxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F320)T cells KO
SA332986Norm. Area 088_0009_01B_R_TM49xWTxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F321)T cells KO
SA332987Norm. Area 088_0009_01B_R_TM50xWTxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F322)T cells KO
SA332988Norm. Area 088_0009_01B_R_TM47xWTxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F319)T cells KO
SA332989Norm. Area 088_0009_01B_R_TM45xKOxCMLxTAx24x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F317)T cells KO
SA332990Norm. Area 088_0009_01B_R_TM43xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F315)T cells KO
SA332991Norm. Area 088_0009_01B_R_TM44xKOxCMLxTAx24x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F316)T cells KO
SA332992Norm. Area 088_0009_01B_R_TM51xWTxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F323)T cells KO
SA332993Norm. Area 088_0009_01B_R_TM46xKOxCMLxTAx24x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F318)T cells KO
SA332994Norm. Area 088_0009_01B_R_TM53xKOxCMLxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F325)T cells KO
SA332995Norm. Area 088_0009_01D_R_TM21xWTxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F193)T cells KO
SA332996Norm. Area 088_0009_01D_R_TM20xWTxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F192)T cells KO
SA332997Norm. Area 088_0009_01D_R_TM19xWTxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F191)T cells KO
SA332998Norm. Area 088_0009_01D_R_TM22xWTxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F194)T cells KO
SA332999Norm. Area 088_0009_01B_R_TM56xKOxCMLxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F328)T cells KO
SA333000Norm. Area 088_0009_01B_R_TM42xKOxCMLxTFx5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F314)T cells KO
SA333001Norm. Area 088_0009_01B_R_TM54xKOxCMLxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F326)T cells KO
SA333002Norm. Area 088_0009_01B_R_TM55xKOxCMLxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F327)T cells KO
SA333003Norm. Area 088_0009_01B_R_TM52xKOxCMLxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F324)T cells KO
SA333004Norm. Area 088_0009_01B_R_TM41xKOxCMLxTFx4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F313)T cells KO
SA333005Norm. Area 088_0009_01C_R_TM51xWTxCMLxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F135)T cells KO
SA333006Norm. Area 088_0009_01C_R_TM50xWTxCMLxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F134)T cells KO
SA333007Norm. Area 088_0009_01C_R_TM49xWTxCMLxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F133)T cells KO
SA333008Norm. Area 088_0009_01B_R_TM28xKOxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F300)T cells KO
SA333009Norm. Area 088_0009_01B_R_TM27xKOxTAx72x4xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F299)T cells KO
SA333010Norm. Area 088_0009_01B_R_TM24xKOxTAx72x1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F296)T cells KO
SA333011Norm. Area 088_0009_01B_R_TM25xKOxTAx72x2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F297)T cells KO
SA333012Norm. Area 088_0009_01B_R_TM26xKOxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F298)T cells KO
SA333013Norm. Area 088_0009_01D_R_TM28xKOxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F200)T cells KO
SA333014Norm. Area 088_0009_01D_R_TM27xKOxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F199)T cells KO
SA333015Norm. Area 088_0009_01B_R_TM38xKOxCMLxTFx1xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F310)T cells KO
SA333016Norm. Area 088_0009_01B_R_TM39xKOxCMLxTFx2xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F311)T cells KO
SA333017Norm. Area 088_0009_01B_R_TM40xKOxCMLxTFx3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F312)T cells KO
SA333018Norm. Area 088_0009_01D_R_TM23xWTxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F195)T cells KO
SA333019Norm. Area 088_0009_01D_R_TM24xKOxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F196)T cells KO
SA333020Norm. Area 088_0009_01D_R_TM26xKOxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F198)T cells KO
SA333021Norm. Area 088_0009_01D_R_TM25xKOxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F197)T cells KO
SA333022Norm. Area 088_0009_01D_R_TM18xKOxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F190)T cells KO
SA333023Norm. Area 088_0009_01D_R_TM17xKOxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F189)T cells KO
SA333024Norm. Area 088_0009_01D_R_TM11xKOxTFx2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F183)T cells KO
SA333025Norm. Area 088_0009_01D_R_TM10xKOxTFx1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F182)T cells KO
SA333026Norm. Area 088_0009_01C_R_TM48xWTxCMLxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F132)T cells KO
SA333027Norm. Area 088_0009_01D_R_TM12xKOxTFx3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F184)T cells KO
SA333028Norm. Area 088_0009_01D_R_TM13xKOxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F185)T cells KO
SA333029Norm. Area 088_0009_01C_R_TM26xKOxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F110)T cells KO
SA333030Norm. Area 088_0009_01C_R_TM27xKOxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F111)T cells KO
SA333031Norm. Area 088_0009_01C_R_TM28xKOxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F112)T cells KO
SA333032Norm. Area 088_0009_01C_R_TM47xWTxCMLxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F131)T cells KO
SA333033Norm. Area 088_0009_01C_R_TM46xKOxCMLxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F130)T cells KO
SA333034Norm. Area 088_0009_01C_R_TM40xKOxCMLxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F124)T cells KO
SA333035Norm. Area 088_0009_01C_R_TM41xKOxCMLxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F125)T cells KO
SA333036Norm. Area 088_0009_01C_R_TM43xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F126)T cells KO
SA333037Norm. Area 088_0009_01C_R_TM39xKOxCMLxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F123)T cells KO
SA333038Norm. Area 088_0009_01C_R_TM38xKOxCMLxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F122)T cells KO
SA333039Norm. Area 088_0009_01C_R_TM45xKOxCMLxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F129)T cells KO
SA333040Norm. Area 088_0009_01C_R_TM44xKOxCMLxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F128)T cells KO
SA333041Norm. Area 088_0009_01C_R_TM25xKOxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F109)T cells KO
SA333042Norm. Area 088_0009_01C_R_TM24xKOxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F108)T cells KO
SA333043Norm. Area 088_0009_01C_R_TM12xKOxTFx3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F96)T cells KO
SA333044Norm. Area 088_0009_01C_R_TM13xKOxTFx4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F97)T cells KO
SA333045Norm. Area 088_0009_01C_R_TM14xKOxTFx5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F98)T cells KO
SA333046Norm. Area 088_0009_01C_R_TM11xKOxTFx2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F95)T cells KO
SA333047Norm. Area 088_0009_01C_R_TM10xKOxTFx1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F94)T cells KO
SA333048Norm. Area 088_0009_01D_R_TM16xKOxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F188)T cells KO
SA333049Norm. Area 088_0009_01D_R_TM15xKOxTAx24x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F187)T cells KO
SA333050Norm. Area 088_0009_01D_R_TM14xKOxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F186)T cells KO
SA333051Norm. Area 088_0009_01C_R_TM15xKOxTAx24x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F99)T cells KO
SA333052Norm. Area 088_0009_01C_R_TM16xKOxTAx24x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F100)T cells KO
SA333053Norm. Area 088_0009_01C_R_TM21xWTxTAx72x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F105)T cells KO
SA333054Norm. Area 088_0009_01C_R_TM22xWTxTAx72x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F106)T cells KO
SA333055Norm. Area 088_0009_01C_R_TM23xWTxTAx72x5xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F107)T cells KO
SA333056Norm. Area 088_0009_01C_R_TM20xWTxTAx72x2xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F104)T cells KO
SA333057Norm. Area 088_0009_01C_R_TM19xWTxTAx72x1xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F103)T cells KO
SA333058Norm. Area 088_0009_01C_R_TM17xKOxTAx24x3xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F101)T cells KO
SA333059Norm. Area 088_0009_01C_R_TM18xKOxTAx24x4xRPpos_15JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F102)T cells KO
SA333060Norm. Area 088_0009_01B_R_TM23xWTxTAx72x5xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F295)T cells KO
SA333061Norm. Area 088_0009_01B_R_TM21xWTxTAx72x3xHILICneg_14JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F293)T cells KO
SA333062Norm. Area 088_0009_01A_R_TM27xKOxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F39)T cells KO
SA333063Norm. Area 088_0009_01A_R_TM28xKOxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F40)T cells KO
SA333064Norm. Area 088_0009_01D_R_TM49xWTxCMLxTAx72x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F222)T cells KO
SA333065Norm. Area 088_0009_01A_R_TM26xKOxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F38)T cells KO
SA333066Norm. Area 088_0009_01A_R_TM25xKOxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F37)T cells KO
SA333067Norm. Area 088_0009_01A_R_TM22xWTxTAx72x4xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F34)T cells KO
SA333068Norm. Area 088_0009_01A_R_TM23xWTxTAx72x5xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F35)T cells KO
SA333069Norm. Area 088_0009_01A_R_TM24xKOxTAx72x1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F36)T cells KO
SA333070Norm. Area 088_0009_01D_R_TM48xWTxCMLxTAx72x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F221)T cells KO
SA333071Norm. Area 088_0009_01D_R_TM47xWTxCMLxTAx72x1xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F220)T cells KO
SA333072Norm. Area 088_0009_01D_R_TM41xKOxCMLxTFx4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F214)T cells KO
SA333073Norm. Area 088_0009_01A_R_TM38xKOxCMLxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F49)T cells KO
SA333074Norm. Area 088_0009_01A_R_TM39xKOxCMLxTFx2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F50)T cells KO
SA333075Norm. Area 088_0009_01D_R_TM43xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F215)T cells KO
SA333076Norm. Area 088_0009_01D_R_TM43xKOxCMLxTFx5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F216)T cells KO
SA333077Norm. Area 088_0009_01D_R_TM46xKOxCMLxTAx24x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F219)T cells KO
SA333078Norm. Area 088_0009_01D_R_TM45xKOxCMLxTAx24x3xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F218)T cells KO
SA333079Norm. Area 088_0009_01D_R_TM44xKOxCMLxTAx24x2xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F217)T cells KO
SA333080Norm. Area 088_0009_01A_R_TM21xWTxTAx72x3xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F33)T cells KO
SA333081Norm. Area 088_0009_01A_R_TM20xWTxTAx72x2xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F32)T cells KO
SA333082Norm. Area 088_0009_01D_R_TM51xWTxCMLxTAx72x5xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F224)T cells KO
SA333083Norm. Area 088_0009_01D_R_TM50xWTxCMLxTAx72x4xRPneg_16JUN23_CELL_HUM_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F223)T cells KO
SA333084Norm. Area 088_0009_01A_R_TM10xKOxTFx1xHILICpos_08JUN23_CELL_MOU_LUM_0000_04AB_15MIN_BLL_KP_01.raw (F22)T cells KO
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Collection:

Collection ID:CO003199
Collection Summary:Tcells from miR142 WT and KO BCR ABL mice were isolated and subjected to resting or 24h activation.
Sample Type:T-cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR003215
Treatment Summary:CD3+ T cells were sorted by flow cytometry from the spleen of Mir142−/−BCR-ABL (KO-T, n=9) and Mir142+/+BCR-ABL (WT-T, n=9) mice (BCR-ABL expression was induced for 2 weeks by tetracycline withdrawal). T cells from 5 mice per group (6x106/sample) were snap-frozen in liquid nitrogen as resting T cells. T cells from 4 mice per group were cultured in the presence of activating anti-CD3/CD28 antibody-coated beads for 24 hours before snap freezing (3x106/sample, activated). Metabolite extraction was performed on resting (6x106/sample, n=5 sample per group) and activated (3x106/sample, n=4 sample per group) T cells from both Mir142−/−BCR-ABL and Mir142+/+BCR-ABL mice and subjected to untargeted metabolomics as described previously.

Sample Preparation:

Sampleprep ID:SP003212
Sampleprep Summary:Metabolite extraction on T cells were performed using 70% acetonitrile/methanol and 30% water. The metabolite extracts were subjected to untargeted metabolomics using HILIC and reversed phase chromatographies in positive and negative modes.
Processing Storage Conditions:4℃
Extraction Method:Polar solvent extraction
Extract Storage:-80℃
Sample Derivatization:none

Combined analysis:

Analysis ID AN005056 AN005057 AN005058 AN005059
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) Thermo Hypersil Gold C18 (150x 2.1mm, 3uM) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak area Peak area Peak area Peak area

Chromatography:

Chromatography ID:CH003819
Chromatography Summary:Reversed phase chromatography
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Thermo Hypersil Gold C18 (150x 2.1mm, 3uM)
Column Temperature:45
Flow Gradient:0%A for 1 min, 50% B for 4 min, 98%B for 3 min, 0% A for 5min
Flow Rate:0.3ml/min
Injection Temperature:4
Solvent A:Water, 0.1% formic acid
Solvent B:Acetonitrile, 0.1% formic acid
Transferline Temperature:275
Target Sample Temperature:4
Chromatography Type:Reversed phase
  
Chromatography ID:CH003820
Chromatography Summary:HILIC chromatography
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:99%B for 1min, 85%B for 2min, 75%B for 3 min, 30%B for 3 min, 99%B for 5 min
Flow Rate:0.35ml/min
Injection Temperature:4
Solvent A:10mM ammonium acetate, 95% Water, 5% Acetonitrile, 0.1% acetic acid
Solvent B:10mM ammonium acetate, 95% Acetonitrile, 95% Water, 0.1% acetic acid
Transferline Temperature:275
Target Sample Temperature:4
Chromatography Type:HILIC

MS:

MS ID:MS004794
Analysis ID:AN005056
Instrument Name:Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:POSITIVE
  
MS ID:MS004795
Analysis ID:AN005057
Instrument Name:Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:NEGATIVE
  
MS ID:MS004796
Analysis ID:AN005058
Instrument Name:Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:POSITIVE
  
MS ID:MS004797
Analysis ID:AN005059
Instrument Name:Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Xcalibur software for Data dependent acquisition Compound discoverer 3.2 for data processing Skyline for data evaluation
Ion Mode:NEGATIVE
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