Summary of Study ST003142
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001953. The data can be accessed directly via it's Project DOI: 10.21228/M88M77 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003142 |
Study Title | Effect of liver Acox1 knockout on serum lipidome in mice |
Study Summary | The liver gene expression of the peroxisomal β-oxidation enzyme acyl-coenzyme A oxidase 1 (ACOX1), which catabolizes very long chain fatty acids (VLCFA), increases in the context of obesity. To check if liver peroxisomal fatty acids beta-oxidation deficiency will affect whole body metabolic homeostasis through circulating lipids. We analyzed serum samples from 5 WT and 5 Acox1-LKO mice. |
Institute | Washington University in St. Louis |
Last Name | Lu |
First Name | Dongliang |
Address | 660 S. Euclid Ave. |
ludong-liang@wustl.edu | |
Phone | 3147476766 |
Submit Date | 2024-03-19 |
Analysis Type Detail | LC-MS |
Release Date | 2024-03-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005155 | AN005156 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Bruker Impact HD | Bruker Impact HD |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH003903 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 42 °C |
Flow Gradient: | t = 0 min, 5% B; t = 10 min, 40% B; t = 18.8 min, 98% B; t = 20.5 min, 98% B. |
Flow Rate: | 250 μL/min. |
Solvent A: | 50% Methanol/40% Acetonitrile/10% Water; 10 mM Ammonium formate |
Solvent B: | 95% Isopropyl alcohol/5% water; 10 mM Ammonium formate |
Chromatography Type: | Reversed phase |