Summary of Study ST003142
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001953. The data can be accessed directly via it's Project DOI: 10.21228/M88M77 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003142 |
| Study Title | Effect of liver Acox1 knockout on serum lipidome in mice |
| Study Summary | The liver gene expression of the peroxisomal β-oxidation enzyme acyl-coenzyme A oxidase 1 (ACOX1), which catabolizes very long chain fatty acids (VLCFA), increases in the context of obesity. To check if liver peroxisomal fatty acids beta-oxidation deficiency will affect whole body metabolic homeostasis through circulating lipids. We analyzed serum samples from 5 WT and 5 Acox1-LKO mice. |
| Institute | Washington University in St. Louis |
| Last Name | Lu |
| First Name | Dongliang |
| Address | 660 S. Euclid Ave. |
| ludong-liang@wustl.edu | |
| Phone | 3147476766 |
| Submit Date | 2024-03-19 |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-03-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005155 | AN005156 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Impact HD | Bruker Impact HD |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
MS:
| MS ID: | MS004891 |
| Analysis ID: | AN005155 |
| Instrument Name: | Bruker Impact HD |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:2500 cts for positive ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0 |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 4200 V |
| Collision Energy: | 10-70 eV |
| Dry Gas Flow: | 5.0 L/min |
| Dry Gas Temp: | 240°C |
| Nebulizer: | 1.2 Bar |
| MS ID: | MS004892 |
| Analysis ID: | AN005156 |
| Instrument Name: | Bruker Impact HD |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:1500 cts for negative ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0 |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 4200 V |
| Collision Energy: | 10-70 eV |
| Dry Gas Flow: | 5.0 L/min |
| Dry Gas Temp: | 240°C |
| Nebulizer: | 1.2 Bar |
