Summary of Study ST000591
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000591 |
Study Title | Metablomic profiling in acc1-5 mutant and wild type arabidiopsis |
Study Summary | This experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites. |
Institute | Agriculture and Agri-Food Canada |
Department | London Research and Development Centre |
Laboratory | Renaud |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford street, London, Ontario, Canada |
justin.renaud@agr.gc.ca | |
Phone | 519-953-6698 |
Submit Date | 2017-03-12 |
Publications | Chen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1. |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-25 |
Release Version | 1 |
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Collection:
Collection ID: | CO000608 |
Collection Summary: | Arabidopsis seeds obtained from the Arabidopsis Biological Resource Center (ABRC) at the Ohio State University were grown in half strength of Murashige and Skoog (½ MS) medium (0.5XMS salts, 1.5% [w/v] sucrose, and 0.8% agar [pH 5.8]) or soil under 16h/8h light/dark cycle at 23°C. Plants were harvested 12 days after germination. 50 mg of each plant (7 mutants, 7 wild type) were ground in liquid N2 using ball mill and immediately extracted following collection protocol. |
Collection Protocol Filename: | collection_protocol.pdf |
Sample Type: | Seedlings |
Collection Location: | London, Ontario |
Collection Frequency: | Single |