Summary of Study ST000591
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000591 |
Study Title | Metablomic profiling in acc1-5 mutant and wild type arabidiopsis |
Study Summary | This experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites. |
Institute | Agriculture and Agri-Food Canada |
Department | London Research and Development Centre |
Laboratory | Renaud |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford street, London, Ontario, Canada |
justin.renaud@agr.gc.ca | |
Phone | 519-953-6698 |
Submit Date | 2017-03-12 |
Publications | Chen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1. |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-25 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000621 |
Sampleprep Summary: | 50 mg of 12 DAG WT and acc1-5 seedlings were collected and grinded in liquid N2 using a ball-mill. The fine powders were suspended in 1 mL ice cooled methanol: water (4:1) by vortex. The mixtures were sonicated in water bath sonicator for 15 mins and followed by centrifugation at 11,000g for 10 mins at 4 °C. 700 µL of the supernatant was transferred into fresh tubes and evaporated to dryness using a vacufuge at ambient temperature. The residue was re-dissolved in 1:1 mixture of methanol: water and vortexed vigorously. All samples were filtered using 0.2 μm PTFE syringe filter (Whatman) and 5 µL of 1 µg/mL 13C6 phenylalanine internal standard (Cambridge Isotopes, Tewksbury, USA) were added to all samples |