Summary of Study ST003131
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001945. The data can be accessed directly via it's Project DOI: 10.21228/M89M7J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003131 |
| Study Title | Untargeted Metabolomics on mouse caecal contents |
| Study Type | Mouse |
| Study Summary | While there is strong evidence for interactions between the microbiota-gut-brain axis and host physiology in the context of chronic stress, limited research has investigated the role of the microbiome in host response to acute stress. Determining the underlying mechanisms by which stress-induced microbiota changes may provoke functional changes in the gut and brain is critical for developing future therapeutics to alleviate the adverse consequences of traumatic stress. Here, we aimed to identify a biological signature of gut metabolites that are significantly altered following exposure to acute restraint stress. Adult male C57Bl/6 conventional, germ-free and colonized germ-free mice underwent a 15-minute restraint stress exposure. Caecal contents were collected from naïve mice and stressed mice, either immediately or 45 minutes following stress. Caecal contents underwent untargeted metabolomics analysis. |
| Institute | University College Cork |
| Department | Psychiatry |
| Laboratory | Microbiota-Gut-Brain Axis Group |
| Last Name | Clarke |
| First Name | Gerard |
| Address | Gaol Walk, Cork |
| G.Clarke@ucc.ie | |
| Phone | +353-21-4901408 |
| Submit Date | 2024-03-19 |
| Num Groups | 9 |
| Total Subjects | 63 |
| Num Males | 63 |
| Analysis Type Detail | Other |
| Release Date | 2024-03-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO003241 |
| Collection Summary: | The acute restraint stress procedure was performed using a clean perforated polypropylene screw-cap 50 mL conical tubes. Cages were randomly assigned to either non-stress or stress groups. Each mouse that underwent stress was placed into the 50 mL tube and restrained for 15 minutes. After 15 minutes of restraint stress, mice were removed from the restrainer and either transported immediately to the cull room or returned to their home cage and left undisturbed for 45 minutes. To control for the variable of transport stress, mice from the non-stress control group were placed into new cages containing fresh bedding and transported the same distance before entering the cull room. Upon entering the cull room, mice were immediately decapitated, trunk blood collected, and tissues harvested. Caecal contents and intestinal samples were manually dissected and stored in PCR-grade microfuge tubes at -80°C until analyses. |
| Sample Type: | Cecum |
| Storage Conditions: | -80? |
