Summary of Study ST001895
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001188. The data can be accessed directly via it's Project DOI: 10.21228/M84X4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001895 |
| Study Title | NMR Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome (Experiment 1) |
| Study Type | 1H NMR Metabolomics to compare toxic and non-toxic species |
| Study Summary | Metabolomics comparison of toxic and non-toxic species of phytoplankton from the genus Alexandrium.This study was carried out using 2 pairwise experiments, A. catenella compared to A. tamarense (Experiment 1) and A. pacificum compared to A. tamarense (Experiment 2). This study includes the NMR data from Experiment 1. |
| Institute | Georgia Institute of Technology |
| Department | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
| Laboratory | Kubanek Lab |
| Last Name | Brown |
| First Name | Emily |
| Address | 950 Atlantic Dr Atlanta, Georgia USA 30332 |
| julia.kubanek@biosci.gatech.edu | |
| Phone | 404-894-8424 |
| Submit Date | 2021-07-20 |
| Num Groups | 2 |
| Total Subjects | 30 |
| Study Comments | Part 1 of 3. This part includes NMR analysis of non-polar metabolites from Experiment 1 using oPLSDA. Parts 2 and 3 inlcude NMR analysis of non-polar metabolites from Experiment 2 and the corresponding mass spectrometry metabolomics for both Experiments 1 and 2. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2021-08-09 |
| Release Version | 1 |
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Project:
| Project ID: | PR001188 |
| Project DOI: | doi: 10.21228/M84X4Z |
| Project Title: | Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome |
| Project Summary: | Recognition and rejection of chemically defended prey is critical to maximizing fitness for predators. Paralytic shellfish toxins (PSTs) which strongly inhibit voltage-gated sodium channels in diverse animal taxa are produced by several species of the bloom-forming algal genus Alexandrium where they appear to function as chemical defenses against grazing copepods. Despite PSTs being produced and localized within phytoplankton cells, some copepods distinguish toxic from non-toxic prey, selectively ingesting less toxic cells, in ways that suggest cell surface recognition perhaps associated with non-polar metabolites. In this study LC/MS and NMR-based metabolomics revealed that the non-polar metabolomes of two toxic species (Alexandrium catenella and Alexandrium pacificum) vary considerably from their non-toxic congener Alexandrium tamarense despite all three being very closely related. Toxic and non-toxic Alexandrium spp. were distinguished from each other by metabolites belonging to seven lipid classes. Of these, 17 specific metabolites were significantly more abundant in both toxic A. catenella and A. pacificum compared to non-toxic A. tamarense suggesting that just a small portion of the observed metabolic variability is associated with toxicity. Future experiments aimed at deciphering chemoreception mechanisms of copepod perception of Alexandrium toxicity should consider these metabolites, and the broader lipid classes phosphatidylcholines and sterols, as potential candidate cues. |
| Institute: | Georgia Institute of Technology |
| Last Name: | Brown |
| First Name: | Emily |
| Address: | 950 Atlantic Dr Atlanta GA 30332, USA |
| Email: | julia.kubanek@biosci.gatech.edu |
| Phone: | 404-894-8424 |
