Summary of Study ST001895
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001188. The data can be accessed directly via it's Project DOI: 10.21228/M84X4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001895 |
Study Title | NMR Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome (Experiment 1) |
Study Type | 1H NMR Metabolomics to compare toxic and non-toxic species |
Study Summary | Metabolomics comparison of toxic and non-toxic species of phytoplankton from the genus Alexandrium.This study was carried out using 2 pairwise experiments, A. catenella compared to A. tamarense (Experiment 1) and A. pacificum compared to A. tamarense (Experiment 2). This study includes the NMR data from Experiment 1. |
Institute | Georgia Institute of Technology |
Department | School of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience |
Laboratory | Kubanek Lab |
Last Name | Brown |
First Name | Emily |
Address | 950 Atlantic Dr Atlanta, Georgia USA 30332 |
julia.kubanek@biosci.gatech.edu | |
Phone | 404-894-8424 |
Submit Date | 2021-07-20 |
Num Groups | 2 |
Total Subjects | 30 |
Study Comments | Part 1 of 3. This part includes NMR analysis of non-polar metabolites from Experiment 1 using oPLSDA. Parts 2 and 3 inlcude NMR analysis of non-polar metabolites from Experiment 2 and the corresponding mass spectrometry metabolomics for both Experiments 1 and 2. |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2021-08-09 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001979 |
Sampleprep Summary: | Non polar extracts were dissolved with volumes calculated to account for different cell densities at the time of harvesting, to a concentration equivalent to 1.62 x 10^7 Alexandrium cells mL-1 in dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane (TMS) as internal standard for 1H nuclear magnetic resonance (NMR) spectroscopy analysis. The extracts were transferred to 3 mm NMR tubes. Extracts from Experiment 1 (A. tamarense-A. catenella) were analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra of non-polar extracts from both experiments were acquired using a Bruker zg30 pulse sequence 1H NMR experiment compiled from 320 scans. Due to poor NMR spectral quality one A. tamarense replicate spectrum was removed from analysis of Experiment 1 (A. tamarense-A. catenella. |
Sampleprep Protocol Filename: | Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome_protocol.pdf |
Extract Storage: | -80℃ |