Summary of Study ST003035
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001888. The data can be accessed directly via it's Project DOI: 10.21228/M8P728 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003035 |
| Study Title | Central Transcriptional Regulator Controls Growth and Carbon Storage under High Light Stress in Photosynthetic Microalgae Model Strains |
| Study Type | Algae |
| Study Summary | Carbon capture efficiency and biochemical storage are some of the primary drivers of photosynthetic productivity and by extension crop yield. To elucidate the mechanisms governing yield phenotypes and carbon allocation regulatory elements, we selected two microalgae strains as simplified models of photosynthetic crops. The Picochlorum celeri TG2 isolate is one of the fastest growing algae and in this work is juxtaposed to a closely related, slower growing, isolate, TG1, of the same species with less than 2% genomic divergence. Through the application of a comprehensive systems biology light-stress response study, we observed a stark difference in carbon assimilation and storage rates, with the slower growing isolate accumulating almost three times the amount of starch compared to the fast-growing isolate. We characterized the carbon storage rates and allocation dynamics, with metabolic bottlenecks, and transport rates of intermediates underlying the variations in growth and composition in high light using instationary 13C-fluxomics experiments. High light stress analysis of transcriptomic dynamics during acclimation of the strains from low to high light identified a widespread response with up to 73% the annotated gene set significantly differentially expressed after only 1 hour. Broad transcriptional regulatory control was inferred by a rapid depletion of a global diel-responsive transcription factor closely related to a circadian-regulator in plants, as the single most distinct transcription factor. Transferring this factor to the slower variant increased yield, specific growth rate, and carbohydrate accumulation of the selected engineered strain, providing further evidence for a coordinating regulatory mechanism for this complex phenotype. |
| Institute | National Renewable Energy Lab |
| Department | Biosciences |
| Laboratory | Laurens Lab |
| Last Name | Laurens |
| First Name | Lieve |
| Address | 15013 Denver West Paekway, Golden, CO 80401 |
| lieve.laurens@nrel.gov | |
| Phone | +1 720-273-6534 |
| Submit Date | 2023-12-18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-26 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003158 |
| Treatment Summary: | Once the 13C pulse was introduced, each identical culture was harvested at different time points, namely 0 (no label pulse), 30, 60, 180, 300, and 600s by rapidly filtering on a 9 cm Fisherbrand glass microfiber filters followed by washing with 15 mL of 0.2 M ammonium bicarbonate in 5% methanol kept in an ice bath and quenching in liquid nitrogen. |
