Summary of Study ST001293
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000875. The data can be accessed directly via it's Project DOI: 10.21228/M8KM4W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001293 |
Study Title | Determining secondary metabolite profile of Ilyonectria spp. pathogenic to ginseng root |
Study Summary | This experiment aims to ascertain a profile of secondary metabolites produced by Ilyonectria species capable of causing disappearing root rot in ginseng. Ilyonectria isolates were grown on potato dextrose agar for 20 days, then plugs were taken from the cultures and extracted with ethyl acetate. Extracts were analyzed by LC-HRMS and tandem HRMS. Data were analyzed by Principal component analysis and molecular networking with GNPS. |
Institute | Agriculture and Agri-Food Canada |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford Street, London, Ontario, LN5V 4T3, Canada |
justin.renaud@agr.gc.ca | |
Phone | 519-953-6698 |
Submit Date | 2019-12-16 |
Total Subjects | 22 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2020-04-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002151 | AN002152 | AN002153 | AN002154 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | Integrated peak area | Integrated peak area | Integrated peak area | Integrated peak area |
Chromatography:
Chromatography ID: | CH001574 |
Chromatography Summary: | Samples were resolved with a reversed-phase column (C-18) |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm,1.8um) |
Column Temperature: | 35 |
Flow Gradient: | 0% B for 0.5 minutes, increased to 100% B over 3 minutes, held at 100% B for 2.5 minutes, decreased to 0% B over 0.5 minutes, then held at 0% B for 1 minute |
Flow Rate: | 0.3 mL/min |
Sample Injection: | 5 uL |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Capillary Voltage: | 3900 V |
Chromatography Type: | Reversed phase |