Summary of Study ST001293

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000875. The data can be accessed directly via it's Project DOI: 10.21228/M8KM4W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001293
Study Title	Determining secondary metabolite profile of Ilyonectria spp. pathogenic to ginseng root
Study Summary	This experiment aims to ascertain a profile of secondary metabolites produced by Ilyonectria species capable of causing disappearing root rot in ginseng. Ilyonectria isolates were grown on potato dextrose agar for 20 days, then plugs were taken from the cultures and extracted with ethyl acetate. Extracts were analyzed by LC-HRMS and tandem HRMS. Data were analyzed by Principal component analysis and molecular networking with GNPS.
Institute	Agriculture and Agri-Food Canada
Last Name	Renaud
First Name	Justin
Address	1391 Sandford Street, London, Ontario, LN5V 4T3, Canada
Email	justin.renaud@agr.gc.ca
Phone	519-953-6698
Submit Date	2019-12-16
Total Subjects	22
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2020-04-15
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001375
Sampleprep Summary:	Cultures were sampled by taking six 0.5-cm plugs from each plate. Plugs were extracted with 4 mL of ethyl acetate, then dried by centrifugal vacuum evaporation. Dried extracts were reconstituted in 1 mL of 3:1 methanol:water and were filtered through 0.45 um PTFE filters (Chromatographic Specialties, Inc.). Composite quality control samples were made by combining 50 uL from each completed extract.

Sampleprep ID:

SP001375

Sampleprep Summary:

Cultures were sampled by taking six 0.5-cm plugs from each plate. Plugs were extracted with 4 mL of ethyl acetate, then dried by centrifugal vacuum evaporation. Dried extracts were reconstituted in 1 mL of 3:1 methanol:water and were filtered through 0.45 um PTFE filters (Chromatographic Specialties, Inc.). Composite quality control samples were made by combining 50 uL from each completed extract.