Summary of Study ST001293
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000875. The data can be accessed directly via it's Project DOI: 10.21228/M8KM4W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001293 |
| Study Title | Determining secondary metabolite profile of Ilyonectria spp. pathogenic to ginseng root |
| Study Summary | This experiment aims to ascertain a profile of secondary metabolites produced by Ilyonectria species capable of causing disappearing root rot in ginseng. Ilyonectria isolates were grown on potato dextrose agar for 20 days, then plugs were taken from the cultures and extracted with ethyl acetate. Extracts were analyzed by LC-HRMS and tandem HRMS. Data were analyzed by Principal component analysis and molecular networking with GNPS. |
| Institute | Agriculture and Agri-Food Canada |
| Last Name | Renaud |
| First Name | Justin |
| Address | 1391 Sandford Street, London, Ontario, LN5V 4T3, Canada |
| justin.renaud@agr.gc.ca | |
| Phone | 519-953-6698 |
| Submit Date | 2019-12-16 |
| Total Subjects | 22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-04-15 |
| Release Version | 1 |
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Project:
| Project ID: | PR000875 |
| Project DOI: | doi: 10.21228/M8KM4W |
| Project Title: | Metabolomic profiling of fungal pathogens responsible for root rot in American ginseng |
| Project Summary: | Ginseng root is an economically valuable crop in Canada at high risk of yield loss caused by the pathogenic fungus Ilyonectria mors-panacis, formerly known as Cylindrocarpon destructans. While this pathogen has been well-characterized from morphological and genetic perspectives, little is known the secondary metabolites it produces and their role in pathogenicity. We used an untargeted LC-MS/MS-based approach paired with global natural products social molecular networking (GNPS) to compare the metabolite profiles of virulent and avirulent Ilyonectria strains. The ethyl acetate liquid culture extracts of 22 I. mors-panacis strains and closely related species were analyzed by LC-MS/MS. Principal component analysis of LC-MS features resulted in two distinct groups, which corresponded to virulent and avirulent Ilyonectria strains. Virulent strains produced more types of compounds than the avirulent strains. The previously reported I. mors-panacis antifungal compound radicicol was present. Additionally, a number of related resorcyclic acid lactones (RALs) were putatively identified, namely pochonins and several additional derivatives of radicicol. Pochonins have not been previously reported in Ilyonectria spp. and have documented antimicrobial activity. This research contributes to our understanding of I. mors-panacis natural products and its pathogenic relationship with ginseng. |
| Institute: | Agriculture and Agri-Food Canada |
| Last Name: | Renaud |
| First Name: | Justin |
| Address: | 1391 Sandford Street, London, Ontario, LN5V 4T3, Canada |
| Email: | justin.renaud@agr.gc.ca |
| Phone: | 519-953-6698 |
