Summary of Study ST002500
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001614. The data can be accessed directly via it's Project DOI: 10.21228/M82T5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002500 |
| Study Title | Plasma metabolomic signatures from patients following high-dose total body irradiation |
| Study Summary | Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure. |
| Institute | Soochow University |
| Last Name | Wang |
| First Name | Chang |
| Address | Suzhou, No. 199, Renai Road, Suzhou Industrial Park |
| wangchang@suda.edu.cn | |
| Phone | +8651265880067 |
| Submit Date | 2023-02-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, raw(Thermo) |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2024-02-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004107 | AN004108 | AN004109 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Thermo TSQ Vantage HPLC-MS/MS | Thermo TSQ Vantage HPLC-MS/MS |
| Column | Agilent DB-5ms (30m x 0.25mm, 0.25um) | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 5977 | Thermo TSQ Vantage | Thermo TSQ Vantage |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | pmoles/l | pmoles/l | pmoles/l |
Chromatography:
| Chromatography ID: | CH003042 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB-5ms (30m x 0.25mm, 0.25um) |
| Column Temperature: | 280 |
| Flow Gradient: | The oven temperature was initially set to 70°C, held for 3min, and then increased to 310°C at a rate of 5°C/min, and finally kept for 10min. |
| Flow Rate: | 1.2 mL/min |
| Solvent A: | N/A |
| Solvent B: | N/A |
| Chromatography Type: | GC |
| Chromatography ID: | CH003043 |
| Chromatography Summary: | positive ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0~1 min, 0.1~10% B; 1-5 min, 10-40% B; 5-17 min, 40-100% B; 17-22min, 100% B; 22-22.1 min, 100-10% B |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | water(0.1% formic acid ) |
| Solvent B: | acetonitrile(0.1% formic acid ) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003044 |
| Chromatography Summary: | negative ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-1min，0.1%B；1-3min，0.1-40%B；3-5min，40-100%B；5-10.5min，100%B；10.5-10.6min，0.1%B；10.6-18min |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | water(5 mM ammonium bicarbonate ) |
| Solvent B: | 95% methanol/water(5mM ammonium bicarbonate ) |
| Chromatography Type: | Reversed phase |
