Summary of Study ST002500

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001614. The data can be accessed directly via it's Project DOI: 10.21228/M82T5P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002500
Study Title	Plasma metabolomic signatures from patients following high-dose total body irradiation
Study Summary	Although some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure.
Institute	Soochow University
Last Name	Wang
First Name	Chang
Address	Suzhou, No. 199, Renai Road, Suzhou Industrial Park
Email	wangchang@suda.edu.cn
Phone	+8651265880067
Submit Date	2023-02-27
Raw Data Available	Yes
Raw Data File Type(s)	cdf, raw(Thermo)
Analysis Type Detail	GC-MS/LC-MS
Release Date	2024-02-28
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002603
Sampleprep Summary:	GC-MS： 200μL of methanol containing 0.5μg/ml tridecanoic acid (IS) was added into 50μL plasma. After vortexmixing, the solution was centrifuged at 13000 rpm for 15 min at 4 ℃. Then the supernatant was transferred into a new eppendorf tube and dried by vacuum (LABCONCO, USA). The dried sample was re-dissolved in 50μL methoxyamine solution (20 mg/mL) for the oximation reaction (37℃,1.5h), which was followed by a silylation reaction with 50μL MSTFA (37℃,1.5h). Finally, the derivatized sample was centrifuged at 13 000 rpm for 15 min and the supernatant was used for the GC-MS analysis. LC-MS： The internal standards (ISs) were dissolved in acetonitrile including d4-choline (d4-CA, 8μg/mL), C19:0 lysoPC (4μg/mL), C17:0-C17:0 PC (8μg/mL), C17:0-C17:0 PE (8μg/mL), d3-C16:0 free fatty acids (d3-C16:0 FFA, 8μg/mL), d4-cholic acid (CA, 4μg/mL) and d4-chenodeoxycholic acid (d4-CDCA, 4μg/mL). For LC-MS analysis, 200 μL ISs was added into 50 μL plasma sample for the protein precipitation. After vortexing, the sample was centrifuged for 10 min (14,000 rpm, 4 ℃), the supernatant (180 μL ) was redissolved with 100μ L 50% methanol before LC-MS analysis.

Sampleprep ID:

SP002603

Sampleprep Summary:

GC-MS： 200μL of methanol containing 0.5μg/ml tridecanoic acid (IS) was added into 50μL plasma. After vortexmixing, the solution was centrifuged at 13000 rpm for 15 min at 4 ℃. Then the supernatant was transferred into a new eppendorf tube and dried by vacuum (LABCONCO, USA). The dried sample was re-dissolved in 50μL methoxyamine solution (20 mg/mL) for the oximation reaction (37℃,1.5h), which was followed by a silylation reaction with 50μL MSTFA (37℃,1.5h). Finally, the derivatized sample was centrifuged at 13 000 rpm for 15 min and the supernatant was used for the GC-MS analysis. LC-MS： The internal standards (ISs) were dissolved in acetonitrile including d4-choline (d4-CA, 8μg/mL), C19:0 lysoPC (4μg/mL), C17:0-C17:0 PC (8μg/mL), C17:0-C17:0 PE (8μg/mL), d3-C16:0 free fatty acids (d3-C16:0 FFA, 8μg/mL), d4-cholic acid (CA, 4μg/mL) and d4-chenodeoxycholic acid (d4-CDCA, 4μg/mL). For LC-MS analysis, 200 μL ISs was added into 50 μL plasma sample for the protein precipitation. After vortexing, the sample was centrifuged for 10 min (14,000 rpm, 4 ℃), the supernatant (180 μL ) was redissolved with 100μ L 50% methanol before LC-MS analysis.