Summary of Study ST003103
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001926. The data can be accessed directly via it's Project DOI: 10.21228/M8RT5W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003103 |
| Study Title | Reinforcing the Evidence of Mitochondrial Dysfunction in Long COVID Patients using a Multiplatform Mass Spectrometry-based Metabolomics Approach |
| Study Summary | Despite the recent and increasing knowledge surrounding COVID-19 infection, the underlying mechanisms of the persistence of symptoms long time after the acute infection are still not completely understood. Here, a multiplatform mass spectrometry-based approach was used for metabolomic and lipidomic profiling of human plasma samples from Long COVID patients (n=40) to reveal mitochondrial dysfunction when compared with individuals fully recovered from acute mild COVID-19 (n=40). Untargeted metabolomic analysis using CE-ESI(+/–)-TOF-MS and GC-Q-MS was performed. Additionally, a lipidomic analysis using LC-ESI(+/–)-QTOF-MS based on an in-house library revealed 471 lipid species identified with high confidence annotation level. The integration of complementary analytical platforms has allowed a comprehensive metabolic and lipidomic characterization of plasma alterations in Long COVID disease that found 46 relevant metabolites which allowed to discriminate between Long COVID and fully recovered patients. We report specific metabolites altered in Long COVID, mainly related to a decrease in the amino acid metabolism and ceramide plasma levels, and an increase in the tricarboxylic acid (TCA) cycle, reinforcing the evidence of an impaired mitochondrial function. The most relevant alterations shown in this study will help to better understand the insights of Long COVID syndrome by providing a deeper knowledge of the metabolomic basis of the pathology. |
| Institute | Universidad CEU San Pablo |
| Department | Chemistry and Biochemistry |
| Laboratory | CEMBIO |
| Last Name | Martinez |
| First Name | Sara |
| Address | Urbanización Montepríncipe, 28660, Boadilla del Monte, Madrid, Spain |
| sara.martinezlopez@ceu.es | |
| Phone | (+34)913724769 |
| Submit Date | 2024-02-15 |
| Num Groups | 2 |
| Total Subjects | 80 |
| Num Males | 14 |
| Num Females | 66 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC/LC-MS |
| Release Date | 2024-03-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005077 | AN005078 | AN005079 | AN005080 | AN005081 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC | CE | CE |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 8890 GC System | Agilent 7100 CE | Agilent 7100 CE |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | GC DB5-MS column (40 m length, 0.25 mm inner diameter, and 0.25 µm film of 95% dimethyl/5% diphenylpolysiloxane) | Agilent Technologies fused silica capillary (total length, 100 cm; internal diameter, 50 µm) | Agilent Technologies fused silica capillary (total length, 100 cm; internal diameter, 50 µm) |
| MS Type | ESI | ESI | EI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Single quadrupole | TOF | TOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 5977B | Agilent 6230 TOF | Agilent 6230 TOF |
| Ion Mode | POSITIVE | NEGATIVE | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | Area | Corrected areas | Corrected areas | Corrected areas | Corrected areas |
Chromatography:
| Chromatography ID: | CH003834 |
| Chromatography Summary: | RP-UHPLC-ESI(+)-QTOF-MS |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 50°C |
| Flow Gradient: | The chromatographic gradient started at 70 % of B at 0 - 1 min, 86 % B at 3.5 - 10 min, 100% B at 11 - 17 min. The starting conditions were recovered by min 17, followed by a 2 min re-equilibration time, reaching a total running time of 19 min |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 10 mM CH3COONH4 and 0.2 mM NH4F in H2O/MeOH (9:1, v/v) |
| Solvent B: | 10 mM CH3COONH4 and 0.2 mM NH4F in ACN/MeOH/IPA (2:3:5, v/v/v) |
| Analytical Time: | 19 min |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003835 |
| Chromatography Summary: | RP-UHPLC-ESI(-)-QTOF-MS |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 50°C |
| Flow Gradient: | The chromatographic gradient started at 70 % of B at 0 - 1 min, 86 % B at 3.5 - 10 min, 100% B at 11 - 17 min. The starting conditions were recovered by min 17, followed by a 2 min re-equilibration time, reaching a total running time of 19 min |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 10 mM CH3COONH4 and 0.2 mM NH4F in H2O/MeOH (9:1, v/v) |
| Solvent B: | 10 mM CH3COONH4 and 0.2 mM NH4F in ACN/MeOH/IPA (2:3:5, v/v/v) |
| Analytical Time: | 19 min |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003836 |
| Chromatography Summary: | GC-Q-MS |
| Instrument Name: | Agilent 8890 GC System |
| Column Name: | GC DB5-MS column (40 m length, 0.25 mm inner diameter, and 0.25 µm film of 95% dimethyl/5% diphenylpolysiloxane) |
| Column Temperature: | The column temperature gradient was programmed as follows: it started at 60 °C for 1 min, then increased by 10 °C/min until reaching 325 °C and held at this temperature for 10 min before cooling down |
| Flow Gradient: | Constant |
| Flow Rate: | 0.5658 mL/min |
| Solvent A: | Helium |
| Solvent B: | N/A |
| Analytical Time: | 37 min followed by 5 min of post-run period |
| Chromatography Type: | GC |
| Chromatography ID: | CH003837 |
| Chromatography Summary: | CE-ESI(+)-TOF-MS |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Agilent Technologies fused silica capillary (total length, 100 cm; internal diameter, 50 µm) |
| Column Temperature: | 20°C |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | BGE (1 M FA in 10% MeOH) |
| Solvent B: | N/A |
| Analytical Time: | 26 min |
| Capillary Voltage: | 30kV |
| Sheath Liquid: | MeOH: H2O (1:1, v/v) with 1mM FA containing two reference masses (purine: 121.0509 and HP-0922: 922.0098) at a flow rate of 0.6 mL/min (1:100 of split ratio) |
| Chromatography Type: | CE |
| Chromatography ID: | CH003838 |
| Chromatography Summary: | CE-ESI(-)-TOF-MS |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Agilent Technologies fused silica capillary (total length, 100 cm; internal diameter, 50 µm) |
| Column Temperature: | 20°C |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | BGE (1 M FA in 10% MeOH) |
| Solvent B: | N/A |
| Analytical Time: | 40 min |
| Capillary Voltage: | -30kV |
| Sheath Liquid: | MeOH: H2O (1:1, v/v) with 1mM FA containing two reference masses (purine: 121.0509 and HP-0922: 922.0098) at a flow rate of 1.0 mL/min (1:100 of split ratio) |
| Chromatography Type: | CE |
