Summary of Study ST002917
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001813. The data can be accessed directly via it's Project DOI: 10.21228/M8C713 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002917 |
| Study Title | Transporter-mediated depletion of apoplastic proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen |
| Study Summary | GC-MS analysis of apoplastic fluid extracted from arabidopsis plants treated with 100 nM flg22 or a mock treatment for 8 hours. Col-0 is wild type arabidopsis plants, QKO is a quadruple knockout mutant in the Col-0 background with knockouts in dde2-2, ein2-1, pad4-1, and sid2-2. |
| Institute | Oregon State University |
| Department | Botany and Plant Pathology |
| Laboratory | Jeff C Anderson |
| Last Name | Rogan |
| First Name | Conner |
| Address | Cordley Hall, 2701 SW Campus Way, Corvallis, OR 97331 |
| roganco@oregonstate.edu | |
| Phone | 3146004945 |
| Submit Date | 2023-08-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-02-28 |
| Release Version | 1 |
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Project:
| Project ID: | PR001813 |
| Project DOI: | doi: 10.21228/M8C713 |
| Project Title: | Transporter-mediated depletion of apoplastic proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen |
| Project Type: | Untargeted GC-MS analysis |
| Project Summary: | In plants and animals, detection of pathogen-associated molecular patterns (PAMPs) by membrane-localized receptors initiates pattern-triggered (PTI) immunity against pathogens. In plants, many PAMP-induced signaling pathways and cellular responses are known, yet how PTI limits pathogen growth is poorly understood. Through a combined metabolomics and genetics approach, we discovered that plant-exuded proline is a virulence-inducing signal and nutrient for the bacterial pathogen Pseudomonas syringae, and that PAMP-induced depletion of proline from the extracellular spaces of Arabidopsis leaves directly contributes to PTI against P. syringae. We further show that PAMP-induced depletion of extracellular proline requires the amino acid transporter Lysine Histidine Transporter 1 (LHT1). This study demonstrates that depletion of a single extracellular metabolite is an effective component of plant immunity. Given the important role for amino acids as N and C sources for microbial growth, their depletion at sites of infection may be a broadly effective defense against many pathogens. |
| Institute: | Oregon State University |
| Department: | Botany and Plant Pathology |
| Laboratory: | Jeff C Anderson |
| Last Name: | Rogan |
| First Name: | Conner |
| Address: | 2701 SW Campus Way, Corvallis, OR, 97330, USA |
| Email: | roganco@oregonstate.edu |
| Phone: | 3146004945 |
