Summary of Study ST002917

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001813. The data can be accessed directly via it's Project DOI: 10.21228/M8C713 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002917
Study TitleTransporter-mediated depletion of apoplastic proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen
Study SummaryGC-MS analysis of apoplastic fluid extracted from arabidopsis plants treated with 100 nM flg22 or a mock treatment for 8 hours. Col-0 is wild type arabidopsis plants, QKO is a quadruple knockout mutant in the Col-0 background with knockouts in dde2-2, ein2-1, pad4-1, and sid2-2.
Institute
Oregon State University
DepartmentBotany and Plant Pathology
LaboratoryJeff C Anderson
Last NameRogan
First NameConner
AddressCordley Hall, 2701 SW Campus Way, Corvallis, OR 97331
Emailroganco@oregonstate.edu
Phone3146004945
Submit Date2023-08-28
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2024-02-28
Release Version1
Conner Rogan Conner Rogan
https://dx.doi.org/10.21228/M8C713
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP003036
Sampleprep Summary:Samples were derivatized with 10 μL of 20 mg/mL methoxyamine hcl in pyridine for 90 minutes at 37 °C. Then 20 μL of MSTFA + 1% TMCS was added and the samples were incubated for 30 minutes at 37 °C. The samples were then randomly injected into the GC-MS.
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