Summary of Study ST002980
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001856. The data can be accessed directly via it's Project DOI: 10.21228/M8T42G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002980 |
| Study Title | Water soluble metabolomics in mice upon loss of SHMT |
| Study Summary | The enzyme SHMT interconverts the amino acids serine and glycine as part of the folate cycle. To explore the role of SHMT in amino acid homeostasis, Mice were treated with a small molecule inhibitor of SHMT (SHIN2) or had Shmt2 genetically knocked-out in a liver specific manner. Serum and liver samples were collected and underwent LC-MS metabolomics analysis. |
| Institute | Princeton University |
| Last Name | McBride |
| First Name | Matthew |
| Address | Carl Icahn Lab, South Drive, Princeton, NJ 08544 |
| matthewmcbride@princeton.edu | |
| Phone | 8567457389 |
| Submit Date | 2023-11-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-12-12 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003099 |
| Sampleprep Summary: | Water soluble metabolites were extracted from serum and liver samples. 3ul of serum was extracted with 120 ul (40X) of 100% methanol, cooled on ice for 10 minutes, centrifuged at 16,000 x g for 30 minutes, and supernatant collected. 20mg of ground liver tissue was extracted with 800ul of 40:40:20 methanol:acetonitrile:water, cooled on ice for 10 minutes, centrifuged at 16,000 x g for 30 minutes, and supernatant collected. |
