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MB Sample ID: SA003733
Local Sample ID: | 88 |
Subject ID: | SU000090 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | approx. 15-16 weeks |
Gender: | male and female |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Feed: | high fat, low fat or normal chow |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000090 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | approx. 15-16 weeks |
Gender: | male and female |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Feed: | high fat, low fat or normal chow |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
88 | SA003733 | FL000941 | obese | Phenotype |
88 | SA003733 | FL000941 | D8 infected | Infection Status |
88 | SA003733 | FL000941 | m | Sex |
Collection:
Collection ID: | CO000073 |
Collection Summary: | - |
Sample Type: | Tissue |
Storage Conditions: | -80 |
Treatment:
Treatment ID: | TR000091 |
Sample Preparation:
Sampleprep ID: | SP000086 |
Sampleprep Summary: | Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol. |
Sampleprep Protocol Filename: | RTI_BECK_Urine-Genetic_HILIC_Metabolomics_Procedure.docx |
Processing Method: | Homogenization |
Extraction Method: | Acetonitrile |
Extract Storage: | -80 C |
Sample Resuspension: | 95:5 Water:Methanol |
Sample Spiking: | L-Tryptophan-d5 |
Organ: | Lung |
Combined analysis:
Analysis ID | AN000111 | AN000112 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 | Waters Synapt G2 |
Ion Mode | POSITIVE | NEGATIVE |
Units | Normalized abundance | Normalized abundance |
Chromatography:
Chromatography ID: | CH000077 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Pressure: | 6000-10000 |
Column Temperature: | 40 C |
Flow Gradient: | Time(min) Flow Rate %A %B: 1. Initial 0.400 99.0 1.0; 2. 1.00 0.400 99.0 1.0; 3. 12.00 0.400 1.0 99.0; 4. 14.00 0.400 1.0 99.0; 5. 15.00 0.400 99.0 1.0; 6. 19.00 0.400 99.0 1.0 |
Flow Rate: | 0.4 mL/min |
Injection Temperature: | 8 C |
Internal Standard: | L-Tryptophan-d5 |
Solvent A: | 50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 50% acetonitrile/50% water; 0.1% formic acid in 10 mM ammonium acetate |
Analytical Time: | 19 min |
Weak Wash Solvent Name: | 5%MeOH |
Weak Wash Volume: | 1000 uL |
Strong Wash Solvent Name: | 80%MeOH |
Strong Wash Volume: | 1000 uL |
Target Sample Temperature: | 8 C |
Sample Loop Size: | 10 uL |
Sample Syringe Size: | 100 uL |
Randomization Order: | Yes |
Chromatography Type: | HILIC |
MS:
MS ID: | MS000087 |
Analysis ID: | AN000111 |
Instrument Name: | Waters Synapt G2 |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 3.2 kV |
Collision Energy: | 4 |
Fragmentation Method: | CID |
Helium Flow: | 180 |
Ionization: | ES+ |
Mass Accuracy: | 10 ppm |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/s |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 75 |
MS ID: | MS000088 |
Analysis ID: | AN000112 |
Instrument Name: | Waters Synapt G2 |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Temperature: | 110 C |
Capillary Voltage: | 2.8 kV |
Collision Energy: | 4 |
Fragmentation Method: | CID |
Helium Flow: | 180 |
Ionization: | ES- |
Source Temperature: | 110 C |
Dataformat: | Continuum |
Desolvation Gas Flow: | 400 L/Hr |
Desolvation Temperature: | 400 C |
Resolution Setting: | 18000 |
Scan Range Moverz: | 50-1000 m/z |
Scanning Cycle: | 1 s |
Tube Lens Voltage: | 74 |